猕猴桃原生质体质膜水通道蛋白特性  

Characterization of Aquaporins at the Plasma Membrane of Leaf Callus Protoplasts from Actinidia deliciosa cv. Hayward

作　　者：邱全胜[1] 王泽宙[1] 蔡起贵[2] 姜荣锡[2] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]中国科学院植物研究所,北京100093

出　　处：《Acta Botanica Sinica》2000年第2期143-147,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金!(39770 0 77)资助项目;国家重点基础研究发展规划项目!(G1 9990 1 1 70 5)&&

摘　　要：采用细胞影像系统 ,测定了猕猴桃 (Actinidiadeliciosacv.Hayward)原生质体转运水分活力。结果表明 ,猕猴桃原生质体在低渗介质中体积迅速增大 ,且随着渗透梯差的加大而显著增大 ;在渗透梯差为 75、10 0、12 5mmol/kg下 ,Pf 值分别为 0 .118× 10 -3 、0 .12 1× 10 -3 、0 .133× 10 -3 cm/s。同时发现猕猴桃原生质体转运水分活力可以被Hg Cl2 抑制 ;并且发现人工通道形成剂两性霉素B能够促进猕猴桃原生质体水分转运 ,表明所测水分转运是通过膜脂双层进行的。实验还发现 ,ZnCl2 和ZnSO4 可以显著抑制猕猴桃原生质体水分转运活力。以上可见 ,猕猴桃原生质体转运水分活力表现出典型的水通道蛋白的特征 。The water transport activity of protoplasts from Actinidia deliciosa cv. Hayward was determined using a cell image system. The results showed that the protoplast volume increased swiftly when the protoplasts were placed in a hypotonic medium, and the volume increased with the increasing osmotic gradients. The P f values were 0.118×10 -3 , 0.121×10 -3 , and 0.133×10 -3 cm/s under the outward osmotic gradients of 75, 100, and 125 mmol/kg, respectively. The results also showed that the water transport activity of protoplasts could be inhibited by HgCl 2 and stimulated by amphotericin B. Moreover, it was found that ZnCl 2 and ZnSO 4 had a significant inhibitory effect on the water transport activity of the protoplasts from A. deliciosa var. deliciosa cv. Hayward. The results indicated that the protoplasts of A. deliciosa var. deliciosa cv. Hayward possessed the typical property of aquaporins, suggesting the presence of aquaporins at its plasma membranes.

关 键 词：猕猴桃 原生质体 质膜 水通道蛋白 细胞影像系统 

分 类 号：Q946.1[生物学—植物学]