肠道病毒71型感染手足口病患儿血浆可溶性人类白细胞抗原-G表达水平的研究  

Expression of plasma sHLA-G in children with hand-foot-and-mouth disease infected with EV71

作　　者：陈晓晴[1] 王慧燕[1] 余玲玲[1] 陈栋[2] 郑晓群[1] 

机构地区：[1]温州医学院附属第二医院检验科,浙江温州325027 [2]温州市疾病预防控制中心微生物检验科,浙江温州325001

出　　处：《中华医院感染学杂志》2013年第11期2530-2532,共3页Chinese Journal of Nosocomiology

基　　金：浙江省重点科技创新团队(2010R50048);浙江省医药卫生科技计划项目(2010KYA137);温州市科技计划项目(Y20100258;Y20100223)

摘　　要：目的通过检测肠道病毒71型(EV71)感染手足口病(HFMD)患儿血浆可溶性人类白细胞抗原-G(sHLA-G)及白细胞介素-6(IL-6)、白细胞介素-10(IL-10)水平,探讨其与儿童EV71感染的关系。方法采用酶联免疫吸附试验(ELISA)检测EV71感染HFMD患儿及正常对照儿童血浆中sHLA-G水平;采用ELISA检测EV71感染HFMD患儿及正常对照儿童的血浆IL-6、IL-10水平;根据卫生部《手足口病诊疗指南》(2010年版)的诊断标准,将患儿组分为普通病例组21例、重型组97例、危重型组19例,同时选取EV71病毒核酸检测阴性的健康体检儿童146例为对照组。结果患儿血浆sHLA-G水平135.1U/ml明显高于正常对照组的19.82U/ml,差异有统计学意义(P<0.01);血浆sHLA-G水平轻型组、重型组、危重型组分别为123.03、118.30、180.20U/ml,差异有统计学意义(P<0.01),其中危重型组的血浆sHLA-G水平高于轻型组和重型组(P=0.000);各组血浆IL-6、IL-10水平差异均有统计学意义(P<0.05),危重型组血浆IL-6、IL-10水平高于重型组和轻型组(Z=-3.057,P=0.002;Z=-2.656,P=0.008)。结论 sHLA-G参与了EV71感染的发生发展过程,血浆sHLA-G水平可作为EV71感染的辅助诊断指标。OBJECTIVE To investigate the plasma soluble HLA-G （s HLA-G） and IL-6 and IL-10 levels in children with hand-foot-and-mouth disease （HFMD） infected with EV71 so as to explore its correlation with the EVT1 infection in children. METHODS The plasma sHLA-G level of the HFMD children infected with EV71 and the normal controls were detected by enzyme linked immunosorbent assay （ELISA）, and the plasma level of IL-6 and IL-10 in the HFMD children infected with EV71 and the normal controls were analyzed by ELISA. According to the guidelines for diagnosis and treatment of hand-foot-mouth disease of the Ministry of Health （2010 edition ）, the children were divided into the normal cases group with 21 cases, the severe cases group with 97 cases, and the critical cases group with 19 cases, meanwhile, 146 healthy children with the EVT1 nucleic acid detected negative were set as the control group. RESULTS The plasma sHLA-G levels in HFMD children infected with EV71 （135.1 U/ml） were dramatically higher than that in normal controls（19.82 U/ml）, the difference was statistically significant （P〈0.01）. The plasma sHLA-G level was 123.03 U/ml in the mild cases group, 118.30 U/ml in the severe cases group ,and 180.20 U/ml in the critical cases group, the difference was statistically significant（P〈 0.01）. The plasma sHLA-G level of the critical cases group was higher than that of both the mild cases group and the severe cases group （P=0. 000）. The differences in the plasma IL-6 level and plasma IL-10 level between the three groups were statistically significant （P〈0.05）. The plasma IL-6 level and plasma IL-10 level of the critical cases group were higher than those of both the mild cases group and the severe cases group（Z= -3. 057, P= 0. 002）Z=-2. 656, P= 0. 008）. CONCLUSION The plasma sHLA-G participates in the progress of EV71 infection in the HFMD children, the plasma sHLA-G might be considered as an index for auxiliary diagnosis of the HFMD.

关 键 词：人类白细胞抗原G 肠道病毒71型 手足口病 白介素-6 白介素-10 

分 类 号：R373[医药卫生—病原生物学]