整体化色谱柱分离测定甘草酸18H-差向异构体  

Separation of the epimers of glycyrrhizic acid with monolithic column

作　　者：邓毓文[1] 杨加兴[1] 杨志达[1] 陈道临[1] 翁文[1] 

机构地区：[1]漳州师范学院化学与环境科学系,福建漳州363000

出　　处：《化学试剂》2013年第6期519-521,共3页Chemical Reagents

基　　金：国家自然科学基金资助项目(20705031);福建省自然科学杰出青年基金资助项目(2012J06005);福建省高等学校新世纪优秀人才支持计划(JK2011030)

摘　　要：建立整体化色谱柱分离分析甘草酸18H-差向异构体的方法。采用C18整体化色谱柱,流动相为磷酸盐缓冲液-乙腈体系,探讨了磷酸盐缓冲液pH及浓度、乙腈含量、柱温等对甘草酸18H-差向异构体分离的影响,在优化色谱条件下,18α-、18β-甘草酸得到基线分离,分离度可达2.75,二异构体出峰时间在10 min内,且在2.12～21.2μg/mL范围内线性关系良好。运用整体化色谱柱缩短了甘草酸18H-差向异构体的分析时间,提高了分析效率,方法可运用于评价甘草酸的异构化及质量控制。A HPLC separation method for the analysis of the epimers of glycyrrhizic acid with a monolithic column was es- tablished. A chromolith RP-18α column was used,the mobile phase was phosphate buffer solution-acetonitrile system. The effects of pH value, phosphate buffer concentration, acetoni- trile content and column temperature on the separation of the epimers were investigated. Under the optimized condition, baseline separation for the two epimers was achieved. The res- olution was 2. 75, and the runtime was 10 minutes. The cali- bration curves were linear in the range of 2. 12 ~ 21.2 μg/ mL. The analysis efficiency was improved with this monolithic column,and this method can be used to track the isomeriza- tion reaction of glycyrrhizic acid.

关 键 词：高效液相色谱法 整体化色谱柱 18Α-甘草酸 18Β-甘草酸 差向异构体 

分 类 号：O657.7[理学—分析化学]