HPLC手性流动相添加剂法拆分盐酸地匹福林对映体  

Enantiomeric separation of dipivefrin hydrochloride by HPLC with chiral mobile phase additives

作　　者：翟明翚[1] 苏立强[1] 王颖[1] 王伟华[1] 周金娟[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《化学试剂》2013年第6期555-557,共3页Chemical Reagents

基　　金：黑龙江省自然科学基金资助项目(B200911)

摘　　要：采用高效液相色谱手性流动相添加剂法对盐酸地匹福林对映体进行了拆分研究。系统考察了手性添加剂的种类及浓度、有机修饰剂的种类及含量、流动相pH和流速等因素对拆分的影响,确定了最佳色谱分离条件:C18色谱柱,流动相为含0.5 g/L CM-β-CD的甲醇-乙腈-水(体积比为15∶70∶15,pH 4.00),流速为0.2 mL/min,检测波长为210 nm。在此条件下,对映体的分离度为2.27。该方法快速、简便、重现性好。The enantiomeric separation of dipivefrin hydro- chloride was invsitigated by HPLC with chiral mobile phase additive. The effects of type and concentration of chiral mobile phase additive,the type and concentration of organic modifi- er,the pH value and the flow rate on the enantiomers separa- tion were investigated systematically. The best separation was obtained with C18 column,the mobile phase consist of metha- nol-aeetonitrile-water, V（methanol） ： V（ aeetonitrile ） ： V（ wa- ter） = 15： 70： 15, containing 0.5 g/L CM-O-CD, pH 4.00, at the flow rate of 0. 2 mL/min. The detection wavelength was set at 210 nm. Under the optimized conditions,the resolution of enantiomers was 2. 27. The method was fast, simple and considerably reproducible.

关 键 词：高效液相色谱 手性流动相添加剂 盐酸地匹福林对映体 

分 类 号：O657.7[理学—分析化学]