三甲基壳聚糖-维生素B_(12)偶联物的制备与表征  

Preparation and characterization of N-trimethyl chitosan-Vitamin B_(12) conjugates

作　　者：柯志洋[1] 程刚[2] 朱晰[1] 何爽[1] 雷英[2] 何斌[2] 黄园[1] 

机构地区：[1]四川大学华西药学院靶向药物与释药系统教育部重点实验室,四川成都610041 [2]四川大学国家生物医学材料工程技术研究中心,四川成都610064

出　　处：《华西药学杂志》2013年第3期243-245,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81173010)

摘　　要：目的合成两种具有肠上皮细胞靶向的三甲基壳聚糖-Vit B12偶联物,并测定Vit B12的修饰度。方法分别以丁二酸和PEG2000为间隔基制备三甲基壳聚糖-Vit B12偶联物。结果与结论合成了两种不同间隔基的三甲基壳聚糖-Vit B12偶联物;目标偶联物及重要中间体经1HNMR、MS和UV确证;两种偶联物中Vit B12的修饰度分别为3.3%、3.1%。OBJECTIVE To synthesize two kinds of intestinal epithelial cells targeting N-trimethyl chitosan-Vit B12 conjugates and determine the level of Vit B12 substitution on the conjugates.METHODS Vit B12 was conjugated with N-trimethyl chitosan via two kinds of spacers：succinic acid and PEG2000.RESULTS and CONCLUSION Two kinds of N-trimethyl chitosan-Vit B12 conjugates were successfully synthesized.The chemical structures of the target conjugations and intermidiates were characterized by 1HNMR,MS and UV.The degree of Vit B12 substitution on the conjugations were 3.3% and 3.1%,respectively.

关 键 词：三甲基壳聚糖 维生素B12 肠上皮细胞靶向 合成 

分 类 号：R94[医药卫生—药剂学]