载As_2O_3白蛋白纳米粒对人K562细胞的增殖抑制作用  

The proliferation inhibition of K562 cells by albumin nanoparticles with As_2O_3

作　　者：于小月[1] 王珍珍[1] 李慧[2] 刘佳宇[1] 李茜[1] 毛声俊[1] 

机构地区：[1]四川大学华西药学院靶向药物及释药系统教育部重点实验室,四川成都610041 [2]四川省医学科学院四川省人民医院血液科,四川成都610072

出　　处：《华西药学杂志》2013年第3期256-258,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家重大新药创制科技重大专项课题(编号:2009ZX09310-002);四川省卫生厅资助项目(编号:303.005.002.280.040)

摘　　要：目的采用白蛋白纳米粒包载As2O3,通过肿瘤细胞摄取载药纳米粒来增强As2O3对K562细胞的增殖抑制作用。方法采用去溶剂化法制备白蛋白纳米粒(ALB-NP),以异硫氰酸(FITC)标记ALB-NP,荧光显微镜观察K562细胞对ALB-NP的摄取;以ALB-NP包载As2O3制备载As2O3白蛋白纳米粒(As2O3-ALB-NP),MTT法比较As2O3与As2O3-ALB-NP对K562细胞增殖抑制率的差异。结果 As2O3-ALB-NP在低浓度(<0.8μmol.L-1)即可显著抑制K562细胞增殖,而As2O3在该浓度对其无抑制作用。结论与As2O3相比,利用ALB-NP载As2O3可显著增强其对K562细胞的增殖抑制作用,有望实现对As2O3用药的增效减毒,为其用于抗肿瘤治疗提供了新的给药策略。OBJECTIVE In order to increase uptake of As2O3 by tumor cells and thus enhance the effect of proliferation inhibition,albumin nanoparticle was chose as a drug carrier to encapsulate As2O3.METHODS Desolvation method was adopt to prepare ALB-NP labeled by FITC.Fluorescence microscopy was made to observe the uptake of ALB-NP（FITC） by K562 cells.Albumin was used to prepare As2O3-ALB-NP with As2O3,and the proliferation inhibition rate of free As2O3 and As2O3-ALB-NP for K562 cells were compared by measuring cell viability using the MTT assay.RESULTS When the concentration of As2O3 was less than 0.8 μmol·L-1,the proliferation inhibition rate of K562 cells in As2O3-ALB-NP group was much higher than that in free As2O3 group.CONCLUSION Compared with As2O3,the As2O3-ALB-NP can significantly enhance the proliferation inhibition rate of K562 cells,which shows that the ALB-NP as a drug carrier of As2O3 can increase the efficiency and reduce the toxicity,and it provides a novel drug administration strategy in antineoplastic therapy.

关 键 词：白蛋白纳米粒 三氧化二砷 白血病 K562细胞 增殖抑制 

分 类 号：R94[医药卫生—药剂学]