HPLC测定假蒟中的α-细辛脑及其含量的动态积累  

Dynamic accumulation research of the content of α-asarone in Piper sarmentosum by HPLC

作　　者：马雯芳[1,2] 余娇[1,3] 蔡毅[1] 闵丹丹[1] 

机构地区：[1]广西中医药大学,广西南宁530001 [2]成都中医药大学,四川成都610075 [3]安吉县妇幼保健院药剂科,浙江安吉313300

出　　处：《华西药学杂志》2013年第3期293-295,共3页West China Journal of Pharmaceutical Sciences

基　　金：广西自然科学基金创新研究团队项目(编号:2011GXNSFF018006);广西科学研究与技术开发计划项目(桂科能1010027-1);广西壮族自治区卫生厅中医药科技专项课题(编号:GZKZ10-034)

摘　　要：目的采用HPLC法测定假蒟中α-细辛脑的含量,观察其动态积累变化。方法色谱柱为phenomenex ODS2柱(250mm×4.60 mm,5μm),流动相为乙腈-0.05%磷酸溶液(49∶51),流速1.0 mL.min-1,检测波长313 nm,柱温30℃。结果α-细辛脑0.0975～1.9500μg与峰面积的线性关系良好(r=0.9998),平均回收率为101.59%(RSD=2.10%,n=6)。结论所用方法简便、快捷、重复性好,可作为假蒟药材中α-细辛脑的含量测定方法。OBJECTIVE To determine α-asarone in Piper sarmentosum Roxb.by HPLC,and observe the changes of dynamic accumulation of α-asarone.METHODS The chromatographic column was C18 ODS2（250 mm×4.60 mm,5 μm）,and the mobile phase was acetonitrile-0.05% H3PO4（49：51）with a flow rate of 1.0 mL·min^-1,the column temperature was 30 ℃ and the detection wavelength was at 313 nm.RESULTS There was a good linear relationship for α-asarone during 0.0975-1.9500 μg（r=0.9998）.The average recovery rate was 101.59%（RSD=2.10%,n=6）.The dynamic accumulation of the content of α-asarone was higher in autumn and winter.CONCLUSION The method is simple,fast and reproducible,and can be used for determination of α-asarone in Piper sarmentosum.

关 键 词：假蒟 Α-细辛脑 HPLC 含量测定 动态积累 

分 类 号：R917[医药卫生—药物分析学]