HPLC测定产舒颗粒中的人参皂苷Rb_1、Re、Rg_1  

Determination of ginsenoside Rg_1,Re,Rb_1 in Chanshu granule by HPLC

作　　者：谢玉[1] 冯俭[2] 席小兰[1] 贝圆[1] 李树翠[1] 

机构地区：[1]成都中医药大学,四川成都610075 [2]成都中医药大学附属医院,四川成都610072

出　　处：《华西药学杂志》2013年第3期313-314,共2页West China Journal of Pharmaceutical Sciences

基　　金：2010年成都市科技局科技攻关计划(编号:10GGYB370SW-023)

摘　　要：目的采用HPLC测定产舒颗粒中人参皂苷Rb1、Re、Rg1的含量。方法采用Diamosil C18色谱柱(250 mm×4.6 mm,5μm),柱温25℃,流动相为乙腈-水、梯度洗脱,流速1 mL.min-1,检测波长203 nm。结果人参皂苷Rb1、Re、Rg1分别在0.424～4.24、0.4312～4.312、0.412～4.12μg,线性关系、回收率、RSD均符合要求。结论所用方法简便、稳定性好、结果可靠。OBJECTIVE To establish an HPLC method for the determination of ginsenoside Rb1,Re,Rg1 in Chanshu granule.METHODS The analytical method was carried out with a Diamosil C18（250 mm×4.6 mm,5μm） at 25 ℃,and the mobile phase consisted of acetonitrile and water for gradient elution with the flow rate at 1.0 mL·min^-1.The detection wavelength was 203 nm.RESULTS The linear ranges for ginsenoside Rbl,Re,Rg1 were 0.424-4.24 mg,0.4312-4.312 mg and 0.41-4.12 mg,respectively.The average recoveries and RSD all met requirements.CONCLUSION The quantitative method for the determination is feasible and beneficial to control the quality of Chanshu granule.

关 键 词：产舒颗粒 人参皂苷RB1 人参皂苷RE 人参皂苷RG1 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]