橡胶树HbFCA启动子的克隆及其在橡胶树中的表达分析  

Cloning and Expression Analysis of the Promoter to HbFCA in Hevea brasiliensis

作　　者：华玉伟[1] 孙芳[1] 黄天带[1] 蔡海滨[1] 杨加伟[1] 黄华孙[1] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737

出　　处：《热带作物学报》2013年第5期800-806,共7页Chinese Journal of Tropical Crops

基　　金：国家天然橡胶产业技术体系(No.nycytx-34);国家自然科学基金(No.31200503)

摘　　要：FCA(Flowering control locus A)基因是自主开花调控途径中的关键调控基因之一,同时,FCA功能具有多样性。本研究中,利用TAIL-PCR方法获得2 039 bp橡胶树FCA基因的启动子序列,生物信息学分析发现该启动子中具有28种顺式调控元件,其中主要为基因表达调控核心元件(TAAT-box和CAAT-box)和光响应调控元件。此外,还含有激素、胁迫信号和光周期的顺式作用元件。同时,将橡胶树FCA启动子与GUS基因融合构建植物表达载体转化橡胶树,发现在胚状体、叶片和树根中GUS基因均强烈表达,但在茎段中表达较弱,说明橡胶树FCA基因除参与开花调控外,还可能参与多种发育过程的调控。本研究获得的HbFCA启动子转化橡胶树植株为进一步分析HbFCA基因功能及表达调控模式提供了遗传材料。Flowering control locus A （FCA）, as a component of the autonomous pathway, is involved in regulating the flowering time in different developmental stage of plant. The promoter of HbFCA of 28 cis-regulation elements with mainly eis-acting element in promoter and enhancer regions （TAAT-box and CAAT-box） and light responsive element was cloned by TAIL-PCR. And GUS gene was strongly expressed in the leaves, roots and somatic embryo in different developmental stage and weakly in the stems in the rubber tree transgenic plant with PHbFCA： GUS. It is indicated that HbFCA plays important role in different developmental stage of rubber tree, and the transgenic plant with PHbFCA：GUS was helpful for the analysis of HbFCA functions.

关 键 词：橡胶树 FCA(Flowering control LOCUS A) 启动子 转基因 

分 类 号：S794.1[农业科学—林木遗传育种]