巴西橡胶树类甜味蛋白基因HbTLP1的克隆及表达分析  

Cloning and Expression Analysis of Gene Encoding Thaumatin-like Protein1 in Hevea brasiliensis

作　　者：闫志烨[1] 秦云霞[2] 罗红丽[1] 

机构地区：[1]海南大学海南省热带作物资源可持续利用重点实验室,海南海口570228 [2]中国热带农业科学院橡胶研究所,海南儋州571737

出　　处：《热带作物学报》2013年第5期860-865,共6页Chinese Journal of Tropical Crops

基　　金：农业部橡胶树生物学重点实验室--海南省热带作物栽培生理学重点实验室开放课题项目(No.KLOF1101);国家自然科学基金项目(No.31160151);教育部高等学校博士学科点专项科研基金课题(No.20114601120003);2011"新世纪优秀人才支持计划"(No.NCET-11-0928);海南省自然科学基金项目No.311030)

摘　　要：根据模式植物拟南芥中同类蛋白的氨基酸序列在橡胶树转录组数据库中进行同源搜索并设计引物,通过RT-PCR方法扩增巴西橡胶树TLP同源基因的cDNA片段,命名为HbTLP1。该片段包含一个984 bp的开放阅读框,编码327个氨基酸的多肽。生物信息学分析结果表明,该氨基酸序列含有一个超级THAUMATIN-Like结构域,与杨树、大豆、葡萄和拟南芥的TPL蛋白有较高的同源性。半定量分析结果表明,该基因的表达受水杨酸(SA)和鞭毛蛋白(flg22)的诱导,但不受茉莉酸(JA)的影响。以上结果揭示该基因为橡胶树的TPL同源基因,参与SA介导的抗病信号途径和植物的先天免疫反应。Based on the result from the amino acid sequence of TLP in Arabidopsis thaliana and the data of RNA-Seq analysis in Hevea brasiliensis, the homolog of rubber TLP was cloned by RT-PCR and named as HbTLP1. The sequencing result showed that the open reading frame of HbTLP1 is 984 bp, encoding 327 amino acids with a conserved THAUMATIN-Like domain similar with TLP from other plants. Bioinformatics analysis showed that the amino acid sequence of the HbTLP1 is similar with that of TLP from Populus tomentosa, Glycine max, Vitis vinifera and A. thaliana.. The expression of HbTLP1 was induced by SA and fig22 at transcript level, but not JA. All the data indicated that HbTLP1 is the TLP homolog in rubber and is related to SA mediated disease resistance signal pathway and involved in plant immunity respond.

关 键 词：巴西橡胶树 类甜味蛋白(TLP) 表达分析 

分 类 号：S794.1[农业科学—林木遗传育种]