木薯褐斑病病原鉴定及其生物学特性研究  

Identification of the Pathogen from Cassava Brown Leaf Spot and Its Biological Characteristics

作　　者：裴月令[1] 时涛[1] 李超萍[1] 蔡吉苗[1] 黄贵修[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带农业有害生物监测与控制重点实验室,海南海口571101

出　　处：《热带作物学报》2013年第5期927-934,共8页Chinese Journal of Tropical Crops

基　　金：国家木薯现代产业技术体系建设项目(No.CARS-12-hnhgx);国家科技支撑计划子课题(No.2007BAD48B06)

摘　　要：褐斑病是中国木薯生产中最为严重的叶部真菌性病害。采用多种分离方法,经致病性评价后获得该病的病原菌,形态观察和ITS序列分析结果表明其为亨宁氏钉孢(Passalora henningsii)。比较光照、菌丝打断、紫外线和菌丝打断-紫外等方法的诱导产孢效果,结果表明:只有菌丝打断-紫外诱导法能够产生大量分生孢子,CME(麦芽木薯叶)培养基、28℃、pH6.0～7.0、紫外线连续诱导、25 mL/皿、14～21 d为产孢最适条件。对菌株CPHHN02的生物学特性研究表明,CA培养基、26～28℃、pH6.0、连续黑暗、碳氮源分别为D-葡萄糖、D-甘露醇、D-乳糖和胰蛋白胨为菌株最适培养条件。大型分生孢子的最适萌发温度为26℃,致死温度为60℃10 min。Brown Leaf Spot is the most serious leaf fungous disease in the cassava plantations of China. In our research, several separation and inoculation methods were applied, pathogenicity test was finished and the pathogen was isolated, morphlogical identification and ITS sequence analysis indicated the pathogen was Passalora henningsii. Four methods（light, hyphae-breaking, UV and hyphae broken-UV）were compared for their inducing capability in producing conidia from P.henningsii. The results showed large number of conidia could obtain by hyphae broken-UV method, and the best condition was Malt-cassava leaves medium, 28 ℃, pH 6.0-7.0, continuous UV induction, 25 mL/plate, 14-21 d. The biological characteristics study of strain CPHHN02 showed CA medium, 26-28℃, pH 6.0, continuous dark, D-glucose, D-mannitol, D-lactose and tryptone were the best culture condition. The optimal temperature for macroconidia germination was 26 ℃ and the lethal temperature was 60 ℃ for 10 min.

关 键 词：木薯 褐斑病菌 生物学特性 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]