低温对椰心叶甲成虫体内几种抗寒物质含量的影响  

Effect of Low Temperature on the Content of Cold-tolerant Substances in the Adult of Brontispa longissima(Gestro)

作　　者：张徐[1] 吕宝乾[2] 金启安[2] 温海波[2] 彭正强[2] 

机构地区：[1]华中农业大学植物科学技术学院,湖北武汉430070 [2]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室海南省热带农业有害生物监测与控制重点实验室,海南儋州571737

出　　处：《热带作物学报》2013年第5期942-946,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31101496);留学人员科技活动择优资助项目(2011年度);973计划子课题(No.2009CB119206);公益性行业计划子课题(No.200903026-5);国家科技合作专项(No.2011DFB30040);海南省自然科学基金(No.312036)

摘　　要：昆虫的抗寒性与其体内抗寒物质的含量密切相关。以短时低温处理椰心叶甲成虫,并以正常饲养的为对照,研究低温对虫体自由水、糖类(总糖、海藻糖、糖原)、甘油、脂肪含量的影响。结果显示:低温诱导处理后椰心叶甲成虫自由水含量急剧下降,由67.10%下降到45.11%,总糖含量略微下降,由19.82μg/mg下降到18.00μg/mg;而海藻糖、糖原、甘油、脂肪4种物质大量积累,其中,海藻糖由3.22μg/mg上升到5.88μg/mg,糖原由1.12μg/mg上升到4.46μg/mg,甘油由11.45μg/mg上升到15.10μg/mg,脂肪由6.43%上升到8.14%。说明椰心叶甲对低温具有一定的应答反应,虫体抗寒物质应答系统为:海藻糖-糖原-甘油-脂肪,虫体有一定的耐寒性。After short-term low temperature treatment on the adults of Brontispa longissima, the impact of low temperature on the content of free water, saccharides（total carbohydrate, trehalose, glycogen）, glycerol and fat in adults was studied. The results revealed the amount of free water was sharply declined from 67.10% to 45.11%, and total carbohydrate was slightly decreased from 19.82（μg/mg）to 18.00（μg/mg）, while the content of trehalose, glycogen, glycerol and fat showed large accumulation compared to the control groups.Trehalose was increased from 3.22 （μg/mg）to 5.88 （μg/mg）;glycogen was increased from 1.12 （μg/mg）to 4.46 （μg/mg） ; glycerol was increased from 11.45 （μg/mg）to 15.10（μg/mg）; fat was increased from 6.43% to 8.14%. The study indicated that B. longissima could defend cold-hardiness and the main antifreezes in adult of B. longissima were trehalose- glycogen-glycogen-fat.

关 键 词：椰心叶甲 低温 自由水含量 糖含量 甘油含量 脂肪含量 

分 类 号：S433[农业科学—农业昆虫与害虫防治]