绿色木霉对膨化玉米秸秆糖化效果的研究  

The research of saccharification about Trichoderma viride on puffed maize straw

作　　者：高晗旸[1] 孙清[1] 孙亮[1] 吕济敏 胡艳清[1] 霍然 

机构地区：[1]沈阳农业大学工程学院,辽宁沈阳110866 [2]甘肃正生生物科技有限公司,甘肃白银730900 [3]浙江中易和节能技术有限公司,浙江杭州310052

出　　处：《可再生能源》2013年第6期75-78,共4页Renewable Energy Resources

摘　　要：以膨化玉米秸秆为原料,利用绿色木霉对膨化玉米秸秆进行糖化处理,研究吐温-80添加量、发酵温度、底物浓度、加酶量、MgSO4添加量对糖化过程的影响。运用二次回归正交组合试验设计,确定出最佳的糖化条件。结果表明,吐温-80添加量为1%(体积分数),温度为36℃,底物浓度为80 g/L,加酶量为每克底物40U,MgSO4添加量为0.4%时,膨化玉米秸秆的糖化效果最佳,糖化率为23.9%。Puffed maize straw treated by Trichoderma viride as raw materials, effects of Tween-80 addition, temperature, substrate concentration, enzyme dosage, MgSO4 during the saccharification process were studied..The optimal saccharification conditions were identified by using quadratic re- gression combination test design. The results show that the best saccharification conditions for puffed maize straw as following： 1% Tween-80 （v/v）, tempreature is 36 ℃, substrate concentration is 80 g/L, enzyme dosage is 40 U/g, MgSO4 is 0.4%. The best glycation rate is 23.9%.

关 键 词：膨化玉米秸秆 绿色木霉 糖化条件 二次回归正交 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]