黄芪总黄酮对肝癌HepG-2细胞凋亡的诱导作用  

TFA Inducing Apoptosis of Human Hepatoma Carcinoma Cell Line HepG-2 and Its Possible Molecular Mechanism

作　　者：张秀云[1] 陈建业[1] 杨映雪[1] 胥正敏[1] 唐华英[1] 

机构地区：[1]川北医学院生化教研室,四川南充637000

出　　处：《中华中医药学刊》2013年第6期1320-1322,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：四川省科技厅重点项目(05SG1859);四川省科技厅应用基础项目(2006J13-031)

摘　　要：目的:探讨黄芪总黄酮(TFA)对人肝癌HepG-2细胞生长抑制及凋亡诱导作用及其机制。方法:以不同浓度TFA处理HepG-2细胞,MTT法测定细胞增殖抑制率;流式细胞仪(FCM)检测细胞凋亡率;Westernblot检测凋亡相关蛋白Survivin、Bc1-2、Bax的表达。结果:TFA能够抑制体外培养的人肝癌细胞HepG-2生长、诱导细胞凋亡,其作用具有量效关系和时效关系。Western blot检测显示,随着TFA浓度的增加,细胞凋亡抑制蛋白Survivin和Bcl-2的表达量明显降低,而细胞凋亡促进蛋白Bax的表达量显著增加。结论:TFA对HepG-2细胞具有抑制增殖和诱导凋亡的作用,其机制与下调细胞凋亡抑制蛋白Survivin与Bcl-2的表达和上调细胞凋亡促进蛋白Bax的表达有关。Objective：To investigate the possible molecular mechanism in TFA inducing apoptosis of human hepatoma carcinoma cell line HepG -2. Methods：HepG -2 ceUs were treated by different concentrations of TFA,the inhibitory rate of cell proliferation was measured by MTI＇. The apoptosis rate was detected by FCM. The expressions of Survivin, Bcl - 2 and Bax protein were detected by Western blot. Results ： TFA can inhibit the proliferation and induce apoptosis of human hepatocellular carcinoma HepG -2 cells,which had a significant effect of time and concentration dependent manner. The expressions of Survivin and Bcl - 2 were decreased, but the expression of Bax was increased. Conclusions ： TFA can inhibit the proliferation and induce apoptosis of HepG -2 cells. The effects may be related to the down - regulation of survivin and Bcl - 2 expressions and up - regulation of Bax expression.

关 键 词：黄芪总黄酮(TFA) 肝癌细胞HEPG-2 细胞凋亡 SURVIVIN BC1-2 BAX 

分 类 号：R96[医药卫生—药理学]