利用灿烂绿褪色法测Fenton体系羟自由基含量  

Brilliant Green Fade Method in Determination of Hydroxyl Radical Content in Fenton System

作　　者：彭彦[1] 张云龙[1] 胡金波[1] 姚立[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2013年第6期1405-1407,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:利用羟自由基使灿烂绿褪色原理,间接检测羟自由基含量。方法:以单因素法通过测量吸光度确定Fenton体系产生羟自由基使灿烂绿褪色的各实验条件;并通过用姜黄素、葛根素、槲皮苷3种物质来验证方法的有效性。结果:通过实验得到实验最佳条件为EDTA-Fe2+(0.945 mmol/L)1 mL、H2O2(3%)2.5 mL、灿烂绿(0.2 mmol/L)1 mL在37℃水浴70 min;根据该体系3种药物清除羟自由基活性姜黄素>葛根素>槲皮苷。结论:灿烂绿褪色法有较好的稳定性;并且能区分姜黄素、葛根素以及槲皮苷的羟自由基清除活性。Objective：To detect the hydroxyl radical levels in Fenton system indirectly based on the principle that hy- droxyl radical can make brilliant green fade. Method： To optimize the brilliant green fade condition using single - factor method,and using three antioxidants：curcumin, puerarin and quercitrin to verify the validity of the method. Results：The optimum conditions for the brilliant green fade system was： EDTA - Fe2＋ （0. 945 mmol/L） 1 mL, H2O2 （3 % ）2.5 mL, brilliant green（0. 2 mmol/L） 1 mL water bath at 37 ℃ for 70 min. The scavenge activity of the three compound was curcumin 〉 puerarin 〉 quercitrin, which was determined by the optimum condition set above. Conclusion：Were established a stable, accurate and reproducible hydroxyl radical determining system, which can distinguish the radical scavenging activity of curcumin, puerarin and quercitrin.

关 键 词：灿烂绿 FENTON体系 羟自由基 

分 类 号：R284.3[医药卫生—中药学]