Notch1信号通路对丹参诱导骨髓基质细胞向神经元样细胞分化的影响  

The role of Notch1 signal-transduction pathway in Salvia miltiorrhiza-induced neuron-like cell differentiation of bone marrow stromal cells

作　　者：黄涛[1] 韩富[1] 张志强[1] 谢海涛[1] 沈有碧[1] 谭齐家[1] 谢才军[1] 伍世表[1] 隋立森[1] 陈捷晗[1] 

机构地区：[1]广东省中医院神经外科,广州510120

出　　处：《广东医学》2013年第9期1322-1326,共5页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:S2011010005403);广东省科技计划项目(编号:粤科函社字[2010]1096号)

摘　　要：目的研究Notch1信号通路对丹参制剂诱导大鼠骨髓基质细胞(BMSCs)向神经元样细胞分化过程的影响。方法取第3代BMSCs,待细胞增殖到60%～70%融合时进行预诱导,预诱导24 h后,更换含100mL/L丹参注射液的DMEM/F12培养液进行诱导分化。待细胞培养0、3、5和7 h,对各组进行形态学观察,RT-PCR和相对定量PCR(qPCR)检测神经元特异性烯醇化酶(NSE)、巢蛋白(Nestin)及Notch1的mRNA表达。结果丹参制剂诱导3 h后,细胞形态发生改变,可见少量突起,诱导培养5 h后细胞突起增多相互交织,呈类神经元细胞分化;诱导7 h后,细胞形态和突起生长情况无明显变化。空白对照组细胞数量有所增加,但细胞形态改变不明显。PCR检测发现,丹参制剂组诱导0、3和5 h,NSE和Nestin的mRNA表达随诱导时间的增加,表达量逐渐增加(P<0.05);诱导7 h,NSE和Nestin的mRNA表达量与5 h相比有所降低,但降低不明显(P>0.05)。丹参制剂组诱导0、3、5和7 h,Notch1的mRNA表达随诱导时间的增加,表达量逐渐降低(P<0.05);同一诱导时间(3、5和7 h)Notch1的mRNA表达量与空白对照组比较降低更加明显(P<0.05)。结论丹参制剂可以促进BMSCs向神经元样细胞分化,且能够抑制Notch1信号通路关键因子Notch1的mRNA表达,提示丹参制剂诱导BMSCs向神经元样细胞分化可能与Notch1信号通路有关。Objective To study the role of extracellular signal - regulated kinase Notch1 signal transduction pathway in Salvia miltiorrhiza - induced neuron - like cells differentiation of rat bone marrow stromal cells （BMSCs）. Metb- otis Rat BMSCs were isolated and purified in vitro, and induced by Salvia mihiorrhiza. Morphologic observation was per- formed 0, 3, 5, and 7 h after induction. The mRNA expression of NSE, Nestin and Notehl was assessed by PCR. Re- suits Some processes on BMSCs were observed 3 hours after induction, and increased processes with network connections were revealed 5 hours after induction. The mRNA expression of NSE and Nestin was significantly increased in a time - de- pendent manner in the first 5 hours after induction （P 〈 0. 05 ）. However, significant reduction of Notehl mRNA was re- vealed in a time - dependent manner after induction （ P 〈 0. 05 ） , also significantly more prominent than that in control group （P 〈 0. 05 ）. Conclusion Salvia miltiorrhiza promotes the neuron - like cell differentiation of BMSCs, also restrains the expression of Notehl, suggesting the involvement of Notchl pathway of this differentiation.

关 键 词：丹参制剂 骨髓基质细胞 Notch1信号通路 神经元样细胞 细胞分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]