辅助菌共培养条件对橙色粘球菌次生代谢产物的影响  

Effects of Co-culture with Helper Bacteria on the Secondary Metabolites of Myxococcus fulvus

作　　者：黄艳[1,2] 胡建伟[1] 朱红惠[2] 

机构地区：[1]塔里木大学塔里木盆地生物资源保护利用兵团重点实验室,阿拉尔843300 [2]广东省微生物研究所广东省华南应用微生物重点实验室培育基地广东省微生物菌种保藏与应用重点实验室广东省微生物新技术公共实验室,广州510070

出　　处：《生物技术通报》2013年第5期184-189,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31170009);广东省自然科学基金项目(10151007002000008);新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题重点项目(BRZD1004)

摘　　要：在常规培养条件下,粘细菌的次级代谢产物含量较低,其研究受到限制。旨在探讨在液态发酵共培养条件下两株辅助菌对橙色粘球菌产生次生代谢产物的影响。结果显示,两株辅助菌的加入可总体上提高次生代谢产物得率。加入42号,43号辅助菌共培养后,次生代谢产物的得率增幅分别为0.05-0.40 g/L和0-0.45 g/L,橙色粘球菌生长至72-84 h接入42号辅助菌得率最大,为0.64 g/L;橙色粘球菌与43号辅助菌同时接入得率最大,为0.69 g/L。但是,两株辅助菌对次级代谢产物组分的影响不同。43号辅助菌的加入能够改变橙色粘球菌的部分次生代谢组分,而42号不能。结果表明,辅助菌与橙色粘球菌的共培养方式能够提高次生代谢产物的得率,有助于粘细菌次生代谢产物的分析。The research of secondary metabolites of myxobacteria was restricted due to it＇s low content under the routine laboratory culture conditions.This study discussed the effect of two helper bacteria to Myxococcus fulvus producing secondary metabolites in liquid fermentation culture conditions.The results showed that the yield and diversity of secondary metabolites were improved by adding the two helper bacteria.After the co-culture of M.fulvus with 42 or 43 helper bacterium,the yield of the secondary metabolites increased from 0.05 g/L to 0.40 g/L and from 0 g/L to 0.45 g/L,respectively.The maximum yield was obtained for 42（0.64 g/L）and 43（0.69 g/L）helper bacterium,when the former was inoculated between 72 to 84 h or the later was cultured with M.fulvus simultaneously.However,the effect of the two helper bacteria on diversity of the secondary metabolites was different.The original secondary metabolites component of M.fulvus was changed by adding 43 helper bacteria but not 42.In conclusion,co-culture with helper bacteria can improve the secondary metabolism of M.fulvus,which would help analyze the secondary metabolites of myxobacteria.

关 键 词：橙色粘球菌 辅助菌 共培养 次生代谢产物 

分 类 号：Q936[生物学—微生物学]