猪导入人类促衰变因子(hDAF)的整合与表达  

Integration and Expression of Exogene hDAF in Transgenic Pigs

作　　者：魏庆信[1] 樊俊华[1] 郑新民[1] 陈付学[1] 熊忠良[1] 华文君[1] 曹军平[1] 李文化[1] 孙方臻[2] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,湖北武汉430209 [2]中国科学院发育生物学研究所,北京100080

出　　处：《中国兽医学报》2000年第3期219-221,共3页Chinese Journal of Veterinary Science

基　　金：国家"8 63"计划资助项目! ( 10 1-0 5 -0 5 -0 1)

摘　　要：将人类促衰变因子 ( human decay accelerating factor,h DAF)基因显微注入 51 7枚猪的受精卵 ,移植到2 6头受体母猪的输卵管内。 2 1头怀孕 ,产仔 93头。产仔后 ,取仔猪的耳或尾组织 ,提取总 DNA。以 5′-TGGCGAGAAGGACTCAGTGATCT-3′和 5′-TGAACTGTTGGTGGGACCTTGGA-3′为引物 ,进行 PCR扩增 ,有 1 8头显示阳性。转基因效率为 3 .5%( 1 8/ 51 7)。对存活的 1 4头转基因猪用免疫荧光法进行检测 ,并采用 MPIAS-50 0多媒体彩色病理图文分析系统进行图像分析 ,其中有 7头的动脉肌肉层中显现有荧光 ,其平均灰度在 1 2 7.3～ 1 70 .The gene of human decay accelerating factor (hDAF) was microinjected into 517 fertilized eggs transplanted into oviducts of 26 donees. 21 pregnant of them produced 93 piglets. Total DNA from ear or tail samples were checked by PCR using primer1 5′ TGGCGAGAAGGCTCAGTGATCT 3′ and primer2 5′ TGAACTGTTGGTGGGACCTTGGA 3′. There were 18 positive piglets with PCR band indicating the transgenic percent of 3.5%. 14 transgenic pigs were analysed by using the fluoroimmunoassay and MPIAS 500, 7 pigs of them revealed the fluorescence in their arterial muscles with the average gray color rate to be indicating 127.3 170.6.

关 键 词：转基因猪 促衰变因子 基因导入 异种器官移植 

分 类 号：R617[医药卫生—外科学] Q78[医药卫生—临床医学]