测定多种动物血清中LH生物活性RRA法的应用  

Utilization of RRA in Bioactive LH Measurement for Variety Animal Species

作　　者：夏国良[1] 付国栋[1] 崔胜[1] 张才乔[1] 吕忠显[1] 王海滨[1] 

机构地区：[1]中国农业大学生物学院,北京100094

出　　处：《中国兽医学报》2000年第3期267-270,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目! ( 3 9670 5 3 7;3 9770 3 96)

摘　　要：利用已建立的可测定多种动物血清中促黄体激素 ( LH)生物学活性的 LH-RRA法 ,检测了不同生理状态下的 LH分泌量 ,进一步分析了该 RRA法在实践应用中的可靠性。通过用特异性刺激 LH分泌的 LRH-A3注射动物和诱导体外培养的垂体细胞分泌 LH的试验表明 ,该方法具有很高的特异性和可靠性。结果显示 ,雄兔对于 LRH-A3刺激有特异性反应。 2只兔前后、不同兔同时的对照试验表明 ,该 RRA法可以特异性地检测出 LRH-A3引起的 LH特异性变化。对母猪发情至早期妊娠阶段血清中 LH检测结果表明 ,此 RRA法可有效地测定出猪血清中 LH的动态变化。利用绵羊垂体细胞体外培养模型 ,分别用不同剂量的 LRH-A3( 0、1、1 0、1 0 0、1 0 0 0 ng/孔 )诱导垂体细胞合成和分泌 LH,RRA测定结果表明 ,试验组 LH含量分别比对照组增加 1 .77、1 .78、2 .0 3和 1 .4 6倍 ( P <0 .0 1 ) ,呈现剂量依赖关系。以上结果表明 ,建立的 LH-h CG的 RRA可以替代Using the established RRA to measure the LH bioactive level of variety animal species, we detected the LH variations of several animals in different physiological conditions to confirm the veracity of RRA results. Preliminary application of LH/hCG RRA to measure serum LH level indicated that the rabbits were specific response to LRH A 3 stimulation and the RRA could specifically measure the LH change induced by LRH A 3. The profiles of swine circulating LH measured by RRA from 2 days before estimated estrous to 25 days early gestation showed that LH/hCG RRA validation to monitor the kinetic LH level. In addition we have established sheep pituitary cell in vitro culture system. Using gradient doses of 0,1,10,100 and 1 000 ng/per well LRH A 3, it induced in vitro culture pituitary cell biosynthesizing and releasing LH. The results showed that the LH level of the experimental groups significantly increased by 1.77, 1.78, 2.03 and 1.46 folds than the control  (P<0.01) and showed a dose dependent relationship of LRH A 3 and LH. The LH/hCG RRA could be a convenient alternative to RIA and the Leydig cell bioassay for monitoring the LH profiles of sera and biological fluids in a variety of species.

关 键 词：促黄体激素 RRA 血清 生物活性 生殖活动 动物 

分 类 号：S852.2[农业科学—基础兽医学] Q575.120.3[农业科学—兽医学]