ABX PENTRA DF 120血液分析仪复检规则建立和评估  

Establishment and evaluation of hematology review criteria for ABX PENTRA DF 120 hematology analyzer

作　　者：吴晓华[1] 林文 柯群[1] 李秋晨[1] 王兆旻 李莉[1] 叶平 

机构地区：[1]上海交通大学医学院附属第一人民医院检验科,上海200080 [2]堀场(中国)贸易有限公司医疗事业部,上海200335

出　　处：《检验医学与临床》2013年第11期1364-1366,共3页Laboratory Medicine and Clinic

摘　　要：目的建立并评价ABX PENTRA DF 120血液分析仪复检规则。方法参考国际血液学复检专家组推荐的"41条"复检规则,结合仪器报警系统,制订暂定复检规则。对本院1 500例标本进行仪器检测和镜检,对暂定规则和仪器提示细胞数量和形态异常报警分别进行统计学评估,制订最终规则;通过分析300例标本,评价最终规则有效性。结果假阳性率最高的前3项暂定规则为未成熟粒细胞、不典型淋巴细胞和血小板聚集。暂定规则形态学评估真阳性率17.47%、假阳性率10.00%、真阴性率69.93%、假阴性率2.6%、复检率27.47%。制订最终规则后,其临床应用复检率为22.00%,经验证未发现血液病理细胞漏检现象。结论本研究制订的ABX PEN-TRA DF 120血液分析仪复检规则具有临床可操作性和有效性。Objective To establish and evaluate the blood smear criteria for ABX PENTRA DF 120 hematology analyzer.Methods Temporary criteria were established by consulting the 41 suggested rules of international consensus group and by using alarm system of ABX PENTRA DF 120.1 500 samples were detected to statistical analyze temporary rule and instrument flag which indicated abnormal cell quantity and morphology.After establishment of review criteria,another 300 samples were tested for validation.Results：Of all rules,instrument flags of ＂LIMC＂,＂ALYM＂ and ＂PAGT ＂ showed most frequent false positive.Results of morphology evaluation showed that true positive rate was 17.5%,false positive rate was 10.00%,true negative rate was 69.9% and false negative rate was 2.6%.According to those results and clinicians opinions,ultimate review criteria for action following automated CBC and leukocytes differential was established.Validated by 300 samples,the rules showed no false negative case.Conclusion The review criteria could be suitable and practical for routine work.

关 键 词：血液分析仪 复检规则 应用评估 

分 类 号：R446.113[医药卫生—诊断学]