向川安瘿蜂和寄生性长尾小蜂共生菌Wolbachia的检测  

The detection of endosymbiotic bacteria Wolbachia in Andricus mukaigawae and Torymus sp.

作　　者：杨筱慧[1] 朱道弘[1] 刘志伟 苏成元[1] 

机构地区：[1]中南林业科技大学昆虫行为与进化生态学实验室,湖南长沙410004 [2]东伊利诺大学生命科学学院,查尔斯顿伊利诺伊州美国61920

出　　处：《湖南农业大学学报（自然科学版）》2013年第3期274-278,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(30872036)

摘　　要：Wolbachia是一类胞质遗传共生菌,能调控被其感染宿主的生殖方式。以向川安瘿蜂虫瘿中获得的向川安瘿蜂和其寄生的长尾小蜂为材料,使用Wolbachia的wsp基因特异引物,通过PCR扩增向川安瘿蜂和长尾小蜂Wolbachia的wsp特异片段,以邻位相连法构建Wolbachia的wsp基因的分子系统树。结果表明:向川安瘿蜂和长尾小蜂均感染了Wolbachia;因二者感染Wolbacia的株系Wa和Wb的wsp序列差异大,不能认定在寄生的长尾小蜂和宿主向川安瘿蜂间存在Wolbachia的水平传播。Wolbachia are maternally inherited endosymbiotic bacteria, and they can manipulate the reproduction of their hosts. In this paper, we amplified the wsp gene fragment of Wolbachia in Andricus mukaigawae and Torymus sp. associated with the plant galls ofA. mukaigawae, by using Wolbachia wsp-specific primers. Our results revealed that A. mukaigawae and Torymus sp. were respectively infected with Wolbachia strain Wa and Wb. We didn＇t found the evidence for horizontal transmission between A. mukaigawae and Torymus sp. because of the difference of wsp sequence in Wolbachia strain Wa and Wb.

关 键 词：向川安瘿蜂 长尾小蜂 WOLBACHIA WSP基因 水平传播 

分 类 号：Q968[生物学—昆虫学]