姜瘟致病菌的分离与鉴定及其拮抗菌的筛选  

The isolation and identification of ginger blast pathogen and screening of its antagonistic fungus

作　　者：王胜利[1] 龚晓[1] 周双德[1] 程鹏[2] 洪亚辉[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南省作物种质创新与资源利用重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2013年第3期282-285,F0003,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：中国烟草总公司湖南省公司资助项目(11-13Aa06)

摘　　要：以湖南省江永县姜瘟病重病区的土壤为材料,对土壤中的主要致病菌进行分离和鉴定,并筛选抑制致病菌的木霉菌。结果表明:姜瘟病重病区土壤中的主要致病菌有2种,分别为G1、H2,其中G1致病性较强,经过菌株形态观察和16S rDNA序列鉴定,发现H2为欧式杆菌(Erwinia spp.),G1为劳尔氏菌(Ralstonia slanacarum);通过对峙实验分析发现,编号为23的哈茨木霉菌(Trichoderma harzianum)对致病菌G1和H2具有较强的拮抗作用,可应用于姜瘟病的生物防治。Major pathogens were isolated and identified from the soil of Jiangyong in Hunan province with serious ginger disease and antagonistic fungus against these pathogens were screened from Trichoderma spp. The results show that two bacterial pathogens G1 and H2 were isolated, of which G1 displayed a stronger pathogenicity. Morphological and 16S rDNA sequence characteristics showed that G1 belonged to Ralstonia slanacarum and H2 belonged to Erwinia spp. The flat confrontation experiment showed that No.23 Trichoderma viride belonged to Trichoderma harzianum had the strongest antagonism to pathogens G1 and H2, which can be used in biological control of ginger disease.

关 键 词：姜瘟病菌 分离 鉴定 拮抗菌 

分 类 号：S632.5[农业科学—蔬菜学] S436.32[农业科学—园艺学]