二甲双胍对卵巢颗粒细胞IRS-1及ERK-2基因表达的影响  

Effect of Metformine on the Expression of Insulin Receptor Substrates-1 and Extracellular Signal Regulated Kinases-2 in Granulosa Cells

作　　者：任文超[1] 刘建新[1] 祁秀娟[1] 丁钰[1] 王琳[1] 

机构地区：[1]青岛大学医学院附属医院生殖中心,266061

出　　处：《国际生殖健康／计划生育杂志》2013年第3期154-156,171,共4页Journal of International Reproductive Health/Family Planning

摘　　要：目的:探讨二甲双胍对多囊卵巢综合征(PCOS)卵巢颗粒细胞胰岛素受体底物-1(IRS-1)及细胞外信号调节激酶-2(ERK-2)的基因表达的影响。方法:收集行体外受精-胚胎移植(IVF-ET)治疗的PCOS患者卵泡颗粒细胞进行体外培养,用二甲双胍处理48 h,采用实时荧光定量聚合酶链反应(PCR)测定颗粒细胞IRS-1及ERK-2基因的相对表达量,另收集行IVF-ET非PCOS患者颗粒细胞作为对照组。结果:PCOS患者颗粒细胞IRS-1基因、ERK-2基因相对表达量与对照组相比明显升高(P<0.05),二甲双胍处理后PCOS患者颗粒细胞IRS-1基因相对表达量升高、而ERK-2基因表达量下降(P<0.05)。结论:PCOS患者颗粒细胞IRS-1与ERK-2基因受二甲双胍作用时表达不同步,二甲双胍可能通过降低PCOS患者颗粒细胞丝裂原活化蛋白激酶(MAPK)信号传导通路活性,减轻MAPK信号通路对磷脂酰肌醇3激酶(PI3K)通路的负反馈作用,改善PCOS患者卵巢胰岛素抵抗状态。Objective： To study the effect of metformoin on the expression of insulin receptor substrate- 1 （IRS-1） and extracellular signal-regulated kinase-2 （ERK-2） in ovarian granulosa cells of the patients with polycystic ovarian syndrome （PCOS）. Methods：Granulosa cells were collected from PCOS patients during IVF- ET. Those granulosa cells from non-PCOS patients during IVF-ET were used as control. After in vitro treated by metformin for 48 h, the cultured granulose ceils were examined for IRS-1 and ERK-2 mRNA expression by real-time quantitative PCR. Results：The expression levels of IRS-1 and ERK-2 mRNAs in the granulosa cells of PCOS patients were higher than those in control group （P〈0.05）. The level of IRS-1 mRNA was increased by metformoin treatment, while the level of ERK-2 mRNA was decreased （P〈0.05）. Conclusions：Mefformoin may alleviate the pathological state of insulin resistance in ovarian of PCOS patients by decreasing the activity of MAPK insulin signaling pathway, which has a negative effect on PI3K pathway.

关 键 词：多囊卵巢综合征 受精 体外 胚胎移植 受体 胰岛素 细胞外信号调节MAP激酶类 

分 类 号：R711.75[医药卫生—妇产科学]