苦豆子ISSR标记的遗传多样性分析  

Genetic diversity analysis on Sophora alopecuroides based on ISSR markers

作　　者：阳翠[1,2] 刘萍[1] 刘姣蓉[1] 郭蒙[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]四川省植物工程研究院,四川内江641200

出　　处：《中草药》2013年第10期1323-1327,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30660017);宁夏大学自然科学基金资助项目(NDZR10-5)

摘　　要：目的通过对产自宁夏、甘肃、青海、新疆和内蒙的苦豆子遗传多样性和亲缘关系的分析,为野生苦豆子资源的驯化和保护等提供依据。方法采用ISSR分子标记技术,对22个苦豆子居群的DNA进行扩增,对其扩增条带进行遗传多样性分析,在所得遗传距离的基础上进行UPGMA聚类和主成分分析(PCA),并绘制亲缘关系树状聚类图。结果 51条ISSR引物共检测到433个位点,每条引物5～12个,平均8.49个;平均多态性位点百分率(PPB)93.30%;Nei’s遗传多样性指数(H)和Shannon信息指数(I)分别为0.335 1、0.499 8;遗传距离变幅范围0.173 6～0.650 2。结论 22个苦豆子居群间具有较高的遗传多样性,各居群间遗传距离与地理距离没有明显关系。Objective To analyze the genetic polymorphism and relationship of Sophora alopecuroides from Ningxia,Gansu,Qinghai,Xinjiang,and Inner Mongolian in China,and to offer some information for the domestication and protection of wild S.alopecuroides germplasms.Methods ISSR primers were used to analyze the genetic diversity of 22 populations of S.alopecuroides.Cluster analysis of the unweighted pair-group method with arithmetic average（UPGMA） and principal component analysis（PCA） were carried out based on the molecular data obtained,and a dendrogram was constructed.Results Totally 433 alleles were amplified using the 51 ISSR primers.The number of allels in per primer range was 5—12,with an average of 8.49.The average percentage of polymorphic loci（PPB） was 93.30%.The Nei’s gene diversity（H） and Shannon’s information index（I） were 0.335 1 and 0.499 8,respectively.The genetic distances range was 0.173 6—0.650 2.Conclusion The genetic polymorphism among 22 populations of S.alopecuroide is relatively abundant and there are no direct relationship between genetic distance and geographical distribution in them.

关 键 词：苦豆子 种质资源 ISSR分子标记 遗传多样性 聚类分析 

分 类 号：R282.12[医药卫生—中药学]