结核性支气管扩张合并真菌感染患者血浆（1—3）-β-D葡聚糖检测分析  

Detection analysis of plasma（1-3）- β -D-glucan in patients with tuberculosis bronchiectasis complicated with fungal infection

作　　者：谢永平 黎国梅 唐雪玲 温杰冉 伦秀红 潘美玉[2] 陈广幸 何嘉颖 

机构地区：[1]广东省肇庆市结核病防治所,广东肇庆526020 [2]广州市胸科医院,广东广州510095

出　　处：《医学检验与临床》2013年第2期32-34,共3页Medical Laboratory Science and Clinics

基　　金：基金项目：广东省肇庆市科技创新计划项目（No20111E389）

摘　　要：目的：探讨结核性支气管扩张合并肺部真菌感染患者进行血浆（1—3）-β-D葡聚糖检测和真菌培养鉴定结果比较其一致性，及其与慢支肺气肿、肺部感染和肺结核等肺部疾病合并真菌感染患者血浆（1—3）-β-D-葡聚糖检测结果进行差异性比较。方法：结核性支气管扩张合并肺部真菌感染患者20例（Ⅰ组）、其他肺部疾病合并真菌感染患者共20例（Ⅱ组），其中包括慢支肺气肿14例、肺部感染5例和肺结核1例，健康对照组20例，应用MB-80微生物动态快速检测系统及真菌（1-3）-β-D葡聚糖检测试剂盒检测三组血浆（1—3）-β-D葡聚糖含量，比较其与真菌培养方法的差异。结果：Ⅰ组、Ⅱ组及对照组血浆（1—3）-β-D葡聚糖含量分别为166．91±74．64pg／ml、164．04±80．53pg／ml和6．05±1．91pg／ml。Ⅰ组与Ⅱ组比较无显著差异（P〉0．05）；Ⅰ组与对照组比较有显著性差异（P〈0．01）。40例肺部疾病合并真菌感染患者血浆（1～3）-β-D葡聚糖阳性，同时行痰液真菌培养法阳性37例（占92．5％）．结论：血浆（1—3）-β-D葡聚糖检测较培养法简便、快速、阳性率高，可用于结核性支气管扩张等肺疾病合并深部真菌感染的早期快速诊断。Objective ： Discussion on patients with tuberculous bronchiectasis pulmonary fungus infection in plasma （1-3） - beta - D glucan, compare the consistency test and fungal culture identification results, and emphysema and chronic bronchitis, pulmonary infection and tuberculosis and other lung diseases with fungal infection in patients with plasma （1-3） - beta - D - glucan difference compared test results. Methods ： Tuberculous 20 patients with bronchiectasis pulmonary fungal infection （ Ⅰ group）, and other lung diseases combined, a total of 20 patients with fungal infection （ Ⅱ group）, including 14 cases of chronic bronchitis emphysema, pulmonary infection in 5 cases and 1 case of tuberculosis, and healthy control group 20 cases, application of MB - 80 dynamic rapid microbial detection system and fungi （1-3） - beta glucan D detection kit （1-3） - three sets of plasma beta - D glucan content and compare the differences and fhngi culture method. Results ： Ⅰ , Ⅱ group and control group in plasma （1-3） - beta - D glucan content were 166.91 ＋ / - 74.64 pg/ml and 164.04 ＋ / - 80.53 pg/ml and 6.05 ＋ / - 1.91 pg/ml. Ⅰ group compared with Ⅱ group no significant difference （P 〉 0.05）; Ⅰgroup compared with control group with significant difference （P 〈 0.01）. 40 cases of pulmonary diseases with fungal infection in patients with plasma （1-3） - beta - D glucan positive, at the same time line sputum positive fungal culture method in 37 cases （92.5%）.Gonclusions ： Plasma （1-3） - beta glucan D detection than culture method is simple, rapid, high positive rate and the tuberculous pulmonary diseases such as bronchiectasis can be used to merge the early rapid diagnosis of deep fungus infection.

关 键 词：(1-3)-β-D葡聚糖 结核性支气管扩张 肺部真菌感染 血浆 

分 类 号：R523[医药卫生—内科学]