地佐辛在雌激素干扰下对切口痛大鼠痛行为学及脊髓c-fos蛋白表达的影响  

作　　者：陈江[1] 程桥[2] 

机构地区：[1]山西医科大学研究生院,山西030001 [2]山西医科大学第一临床医院麻醉科

出　　处：《当代医学》2013年第16期22-23,共2页Contemporary Medicine

摘　　要：目的观察地佐辛在雌激素干扰下对切口痛大鼠脊髓c-fos蛋白表达的影响,探讨雌激素对地佐辛镇痛效果的影响。方法 32只雌性SD大鼠随机分为4组,每组8只,分别为去卵巢手术组、雌激素组、地佐辛组和雌激素+地佐辛组。去卵巢手术组摘除双侧卵巢不做任何处理;雌激素组:在造模前二个小时经皮下注射17β-雌二醇(0.2mg/100g);地佐辛组:在造模后经尾静脉注射地佐辛(0.1mg/100g);雌激素加地佐辛组:在造模前二个小时经皮下注射17β-雌二醇0.2mg/100g;在造模后经尾静脉注射地佐辛0.1mg/100g;采用累积疼痛评分方法对其进行行为学评价,并于术后2h将大鼠处死取其脊髓膨大处,通过酶联免疫吸附实验(ELISA)法研究脊髓背角c-fos蛋白的表达情况。结果与去卵巢手术组比较,地佐辛组,雌激素组,地佐辛+雌激素组累计疼痛评分均下降(P<0.05),同时c-fos表达量也明显减少(P<0.05);同雌激素组比较,地佐辛组以及地佐辛+雌激素组累计疼痛评分以及c-fos表达均明显下降(P<0.05);同地佐辛组比较,地佐辛+雌激素组累计疼痛评分以及c-fos表达差异均无统计学意义(P>0.05)。结论雌激素单独应用对c-fos表达有一定程度抑制作用,但雌激素对地佐辛的镇痛效果没有明显的影响。Objective To investigate the effect of dezocine injection in estrogen interfering and spinal c-fos expression in rat models with incisional pain.Methods Thirty-two female SD rates were randomLy assigned to four groups （n=8）：removal ovary operation group,estrogen group,dezocine group and estrogen and dezocine group. Cumulative pain scoring was performed to evaluate the pain behavior and the rates were killed at 2 hours after operation. The enlargement of spinal cord was harvested to determine the c-los expression by immunohistorchemistry. Results Compared with removal ovary operation group, dezocine group and estrogen and dezocine group had lower cumulative pain scores（P〈 0.05 ）and lower c-los expression（P 〈 0.05 ）.Cumulative pain scores and c-fos expression were significantly lower in dezocine group and estrogen and dezocine group compared with dezocine group （P 〈 0.05 ）.The difference of cumulative pain scores and c-los expression between Dezocine group and estrogen and dezocine group was not statistically significant （P〉0.05）. Conclusion Using estrogen alone can have a certain degree of inhibition to c-fos expression,but estrogen have no sianificant effection to analgesic effect of dezocine.

关 键 词：地佐辛 雌激素 镇痛 原癌基因C-FOS 

分 类 号：R965[医药卫生—药理学]