二参汤不同组成配比对雌性去势大鼠的影响  

Effects of Ershen Decoction with Different Compositions on Ovariectomized Female Rats

作　　者：王丹丹[1] 

机构地区：[1]南京市中医院,江苏南京210001

出　　处：《河南中医》2013年第6期877-880,共4页Henan Traditional Chinese Medicine

摘　　要：目的:观察二参汤(玄参、太子参)的最佳组方剂量。方法:选取60只SD清洁级雌性大鼠随机分为空白对照组、模型组、二参汤(1∶3)大剂量组、二参汤(1∶3)小剂量组、二参汤(2∶3)大剂量组、二参汤(2∶3)小剂量组。通过手术去卵巢的方法造模,空白对照组与模型组给予生理盐水,二参汤(1∶3)大剂量组、二参汤(1∶3)小剂量组、二参汤(2∶3)大剂量组、二参汤(2∶3)小剂量组根据体质量给药,分别给予不同剂量二参汤,持续给药4周。通过观察体征和高效液相法测脑内单胺类递质去甲肾上腺素,五羟色胺,多巴胺含量变化等指标找出最优组合。结果:给予二参汤4周后,摄食量、饮水量和体温二参汤(1∶3)剂量组优于二参汤(2∶3)剂量组,子宫系数和肾上腺系数给药剂量组均未见明显改善。结论:二参汤能改善模型大鼠肾阴虚症状,且二参汤(1∶3)剂量组效果更好。Objective： To observe the best dose of prescription of Ershen Decoction（radix scrophulariae,radix pseudostellariae）.Methods： 60 SD clean female rats were randomly divided into blank control group,model group,high dose group of Ershen Decoction（1∶ 3）,small dose group of Ershen Decoction（1∶ 3）,high dose group of Ershen Decoction（2∶ 3） and small dose group of Ershen Decoction（2∶ 3）.Methods： Model rats were ovariectomized,the blank control group and model group were given normal saline,while high dose group of Ershen Decoction（1∶ 3）,small dose group of Ershen Decoction（1∶ 3）,high dose group of Ershen Decoction（2∶ 3） and small dose group of Ershen Decoction（2∶ 3） were continuously given different dose of Ershen Decoction for 4 weeks according to their body weights.Norepinephrine monoamine neurotransmitters in brain,serotonin,dopamine content were measured by observing the signs and HPLC method to find the optimal composition.Results： After 4 weeks of administration of Ershen Decoction,the food intake,water intake and body temperature in small dose group of Ershen Decoction（1∶ 3） were better than those of high dose group of Ershen Decoction（2∶ 3）,there was no obvious improvement in the uterine coefficient and adrenal coefficient indexes in dosage groups.Conclusion： Ershen Decoction can improve the symptoms of model rats with kidney yin deficiency,and it has better effect in dose groups of Ershen Decoction（1∶ 3）.

关 键 词：二参汤 雌性去势大鼠 单胺类递质 肾阴虚证 绝经综合征 

分 类 号：R285.5[医药卫生—中药学]