疣蝗5个种群间遗传分化的RAPD分析  

Genetic difference among 5 populations of Trilophidia annulata (Thunberg) using RAPD technique

作　　者：汪桂玲[1] 郑哲民[1] 黄原[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《陕西师大学报（自然科学版）》2000年第3期100-103,共4页Journal of Shaanxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目!(39570 110 )

摘　　要：利用随机扩增多态性DNA(RAPD)技术 ,在DNA分子水平上对疣蝗 5个种群间的遗传差异进行了研究 .在 2 3个引物中有 6个引物出现稳定的扩增带 ,扩增总带数为 6 6条 ,其中 5 3条具多态性 ,占 80 3% ,平均每个引物 8 9条 .利用Nei&Li相似系数和UPGMA聚类法对结果进行分析 ,发现用该法检测到的遗传差异从南向北是连续性梯度差异 。The genetic differences of 5 populations of Trilophidia annulata (Thunberg) are compared at DNA level using RAPD method. Of 23 10 base arbitrary primers that are tested, 6 are informative and yielded 66 bands, 53 (80.3%) of which are polymorphs bands with a mean 8.9 bands per primer. Nei′s similarity index and UPGMA are used for analyzing the genetic relationships among 5 populations. The results show that RAPD patterns are species specific and appear continuous variation from south to north populations T. annulata should be a monotypic species in the studied regions.

关 键 词：疣蝗 随机扩增多态性DNA 种群 RAPD 遗传分化 

分 类 号：Q969.265.2[生物学—昆虫学] Q78