ω-3多不饱和脂肪酸对严重烫伤大鼠肝脏及炎症反应的影响  

Influence of ω-3 polyunsaturated fatty acids on liver and inflammatory response in serious scalded rats

作　　者：潘浩斌[1] 蔡晨[1] 李兴照[1] 徐庆连[1] 

机构地区：[1]安徽医科大学第一附属医院烧伤科,合肥230022

出　　处：《安徽医科大学学报》2013年第7期738-741,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:90413267);安徽省卫生厅资助项目(编号:2008A032)

摘　　要：目的观察ω-3多不饱和脂肪酸(ω-3 PUFA)对严重烫伤大鼠肝脏及炎症反应的影响,并就其对肝脏保护作用的机制进行探讨。方法 72只SD大鼠随机分为3组:假烫组8只、对照组(中长链脂肪酸组)32只和治疗组(ω-3 PUFA组)32只。采用大鼠30%总体表面积(TBSA)Ⅲ度烫伤模型。对照组每天按时经尾静脉注射与治疗组相同热量的中长链脂肪酸;治疗组经尾静脉注射ω-3 PUFA 2 ml/(kg.d);假烫组不予任何处置。观察假烫组、对照组和治疗组烫伤后1、4、7、10 d血清内毒素(ET)、前列腺素E2(PGE2)的含量;免疫组化检测肝组织核因子-κB(NF-κB)p65的活性水平;RT-PCR检测肝组织肿瘤坏死因子α(TNF-α)mRNA的表达,各个时间点均处死8只大鼠。结果①在伤后所有时间点,对照组、治疗组血清ET、PGE2水平明显高于假烫组(P<0.01);治疗组血清ET、PGE2水平比对照组降低,伤后4、7、10d与对照组比较,差异有统计学意义(P<0.05)。②伤后对照组、治疗组肝组织NF-κBp65表达比假烫组增强;在伤后4、7 d,与对照组比较,治疗组肝组织NF-κBp65表达减弱(P<0.05)。③对照组、治疗组在烫伤后各时间点,肝组织TNF-αmRNA表达较假烫组明显升高(P<0.01),与对照组比较,在烫伤后4、7 d,治疗组肝组织TNF-αmRNA的表达明显降低(P<0.05)。结论①ω-3 PUFA能降低烫伤后血清ET、PGE2的含量,减轻肝脏组织NF-κBp65和TNF-αmRNA的表达。②ω-3 PUFA减轻严重烫伤后肝组织的损伤。Objective To study the influence of ω-3 polyunsaturated fatty acids （ ω-3 PUFA） on liver and inflam- matory reaction in serious scalded rats and explore its mechanism. Methods A total of 72 SD rats with 30% total body surface area （TBSA） third-degree scalded were optional separated into 3 groups： the phony scald group 8, the control group （middle-long-chain fatty acids group） 32 and treatment group （ω-3 PUFA group）32. Rats in the control group were injected middle-long-chain fatty acids with equivalent calories by tail vein, and rats in the treat- ment group were injected （ω-3 PUFA 2 mL/（ kg · d） by tail vein on time for everyday. The phony scald group,the control group and treatment group 1,4,7,10 d were measured respectively the levels of serum endotoxin （ET）, prostaglandinE2 （ PGE2 ） and expression of nuclear factor-kappa B （ NF-KB ） p65 in liver tissue was detected by im- munohistochemistry staining. Expression of liver tissue TNF-α mRNA was measured by RT-PCR technology. Each subgroup had 8 rats. Results （1） Serum ET, PGE2 were obvious higher than the phony scald group at each time point in the control group and treatment group （P 〈 0. 01 ）. The levels in the treatment group were lower than in the control group, and were significantly different in 4, 7,10 d after scalded （P 〈 0.01 ）. （2） Compared with the phony scald group, the expression of liver tissue NF-KBp65 was increased in the control group and treatment group ; Com- pared with the control group, the activity of NF-kBp65 was significantly decreased after scalded 4, 7 d in the treat- ment group （ P 〈 0. 05 ）. （3） The control group and treatment group at all time points after scalded the expression of liver tissue TNF-a mRNA was significantly higher than the phony scald group（ P 〈0.01 ） ; Compared with the con- trol group, after scalded 4, 7d, the expression of liver tissue TNF-α mRNA was significantly reduced in the treat- ment group （ P 〈 0. 05 ）. Conclusion pression

关 键 词：Ω-3多不饱和脂肪酸 肝脏 烧伤 内毒素 前列腺素E2 核因子-ΚB 

分 类 号：R644[医药卫生—外科学] R575.5[医药卫生—临床医学]