聚桂醇对人肝癌HepG2细胞增殖及迁移影响的体外研究  

The migration and proliferation effects of Lauromacrogol on hepatocellular carcinoma cell in vitro

作　　者：胡中倩[1] 张炽敏[1] 李嘉[1] 李岭[1] 王玲[1] 沈会明[1] 高启[1] 

机构地区：[1]东南大学附属中大医院超声诊断科,南京210009

出　　处：《安徽医科大学学报》2013年第7期772-775,共4页Acta Universitatis Medicinalis Anhui

基　　金：江苏省卫生厅资助课题(编号:H200756)

摘　　要：目的体外探讨聚桂醇对人肝癌HepG2细胞的生长、迁移影响及血管内皮生长因子(VEGF)的表达调控。方法应用MTS比色法观察不同浓度的聚桂醇对人肝癌细胞系细胞的生长抑制作用,并观察细胞在各时间点的抑制率;平板克隆实验检测细胞克隆形成能力的变化;用细胞划痕实验观察对肝癌细胞迁移的影响;Western blot检测VEGF蛋白的表达变化。结果聚桂醇以剂量依赖的方式抑制HepG2细胞生长,0、20、40、60及80μg/ml聚桂醇对肝癌细胞的生长抑制率分别为0、26.78%、35.37%、51.52%和67.15%(P<0.05);聚桂醇能抑制肝癌细胞的克隆形成、迁移及VEGF蛋白表达,且抑制VEGF蛋白表达跟作用时间呈正相关。结论聚桂醇能抑制肝癌细胞增殖及迁移,影响VEGF蛋白表达。Objective To explore the effects of Lauromacrogol on the cell growth , migration and the expression ofvascular endothelial growth factor （VEGF） in HepG2 cells in vitro. Methods The inhibition effects during prolif- eration of HepG2 cells treated with Lauromacrogol were evaluated with MTS assay; cloning formation assay and scratching assay were used to study the effects on proliferation and mobility capability of liver cancer cells; the ex- pression of VEGF was detected with Western blot. Results Lauromacrogol inhibited the growth of HepG2 cells in a dose-dependent manner. The growth inhibitory rates of HepG2 cells treated with 0, 20, 40, 60, and 80 μg/ml Lauromacrogol were 0% ,26. 78% ,35.37% ,51.52% and 67. 15% , respectively （P 〈0. 01 ）. Lauromacrogol sig- nificantly inhibited the growth, migration and down-regulated the expression of VEGF clusion Lauromaerogol inhibits liver cancer cell proliferation, migration and inhibits with time-dependance. Con- the expression of VEGF.

关 键 词：HEPG2细胞 聚桂醇 血管内皮生长因子 细胞增殖 细胞迁移 

分 类 号：R392.3[医药卫生—免疫学]