抗凝剂在改良MAIPA法中对ITP患者血小板特异性自身抗体(GPⅡbⅢa和GPⅠbα)检测的影响  

Influence of the anticoagulant on detection of the ITP anti-platelet GPIIb/IIIa and GPIbα antibodies on based modified MAIPA

作　　者：阮敏[1] 朱凌燕[1] 曾庆曙[1] 夏瑞祥[1] 倪合宇[2] 

机构地区：[1]安徽医科大学第一附属医院血液科,合肥230022 [2]加拿大多伦多大学实验医学与病理生物学系

出　　处：《安徽医科大学学报》2013年第7期832-834,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金项目(编号:1308085MH157);安徽省国际科技合作计划项目(编号:10080703039)

摘　　要：探究二价阳离子络合剂的抗凝剂在改良单克隆抗体俘获血小板抗原(MAIPA)法检测免疫性血小板减少症(ITP)患者血小板特异性自身抗体中的影响。分别留取ED-TA-K2和肝素钠抗凝的162例ITP患者标本,采用改良MAI-PA法检测血小板特异性自身膜糖蛋白IIbIIIa(GPIIbIIIa)和膜糖蛋白Ibα(GPIbα)。抗GPIIbIIIa抗体:单用EDTA-K2抗凝剂检出阳性77例,单用肝素钠抗凝剂检出阳性78例,双重检测阳性102例;抗GPIbα抗体:单用EDTA-K2抗凝剂检出阳性53例,单用肝素钠抗凝剂检出阳性51例,双重检测阳性72例。单一抗凝剂与双重抗凝剂比较差异有统计学意义(P<0.05)。二价阳离子络合剂的抗凝剂在改良MAIPA法检测ITP患者血小板特异性自身抗体中有一定影响,采用两种抗凝剂双重检测可提高自身抗体的检出率。To investigate the influence of differet anticoagulant by modified monoclonal antibody immobilization of platelet antigen assay （MAIPA）, on immune thrombocytopenia （ITP） platelet specific antibodies glycoprotein IIbI- IIα （GPIIbIIIa） and glycoprotein Ibct （GPIbct）. Samples from ethylene diamine tetraacetie acid dipotassium （ED- TA-K2） and heparin anticoagulation patients were obtained. 162 ITP samples were detected by modified MAIPA platelet specific autoantibodies （GPIIb Ⅲ a and GPIbα ）. Group of patients were detected. Single anticoagulants group and dual anticoagulants group differences are statistically significant in detection of GPIIbIIIa and GPIbα（P 〈 0.05 ）. There is significant effect in detection of different anticoagulant detected ITP platelet specific antibodies on modified MAIPA. Adopting both anticoagulants to perform combined detection can improve ITP platelet specific antibodies.

关 键 词：抗凝剂 MAIPA法 血小板 特异性自身抗体 

分 类 号：R558.2[医药卫生—血液循环系统疾病]