鸡肝脏基础型脂肪酸结合蛋白和肝脏脂肪酸结合蛋白配基结合特性分析  

Analysis of Ligand Binding Specificity of Chicken Liver Basic Fatty Acid Binding Protein(Lb-FABP) and Liver Fatty Acid Binding Protein(L-FABP)?

作　　者：赵玉芳[1,2,3] 冷丽[1,2,3] 王守志[1,2,3] 张慧[1,2,3] 王宇祥[1,2,3] 王启贵[1,2,4] 李辉[1,2,3] 

机构地区：[1]农业部鸡遗传育种重点实验室,黑龙江哈尔滨150030 [2]黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,黑龙江哈尔滨150030 [3]东北农业大学动物科学技术学院,黑龙江哈尔滨150030 [4]重庆市畜牧科学院,重庆402460

出　　处：《中国家禽》2013年第10期6-10,共5页China Poultry

基　　金：国家自然科学基金(30972087);现代农业产业技术体系建设专项资金(CARS-42);黑龙江省高等学校科技创新团队建设项目(2010td02)

摘　　要：为探讨鸡肝脏基础型脂肪酸结合蛋白(Lb-FABP)和肝脏脂肪酸结合蛋白(L-FABP)蛋白的配基结合属性,诱导表达并利用Glutathione Sepharose 4B亲和层析纯化了鸡的GST/Lb-FABP和GST/L-FABP融合蛋白,得到的GST/Lb-FABP和GST/L-FABP融合蛋白大小分别为38ku和40ku。将鸡的Lb-FABP和L-FABP分别与1,8-ANS探针结合,结果显示Lb-FABP和L-FABP均表现出对荧光探针的较强结合能力;为进一步测定棕榈酸、花生四烯酸、油酸、胆汁酸和维甲酸这5种配体与Lb-FABP和L-FABP结合的特异性和亲合力,使用这些脂质分子与1,8-ANS探针进行置换分析,结果表明鸡Lb-FABP与维甲酸结合能力最强,而L-FABP与油酸的结合能力最强。本研究结果为深入探究Lb-FABP和L-FABP的生物学功能提供了良好的基础。The aim of this study was to explore the ligand binding property of chicken liver basic fatty acid binding protein （Lb-FABP） and liver fatty acid binding protein （L-FABP）. GST/Lb-FABP and GST/L-FABP were induced and then purified by Glutathione Sepharose 4B,which were 38 ku and 40 ku of molecular weight,respectively. The fluorescent probe 1-anilinonaphthalene 8-sulfonic acid （1, 8-ANS） was used to investigate binding property of Lb-FABP and L-FABP. The results showed that both of Lb-FABP and L-FABP displayed high binding ability. In order to quautitate binding specificity and affinity of palmitie acid, arachidomic acid, oleic acid, cholie acid and retinoid acid with Lb-FABP and L-FABP, a competition binding assay was developed to monitor the ability of these lipid molecules to displace bound 1,8-ANS from the binding cavity. The binding to retinoid acid and L-FABP binding to oleic of the biological function of Lb-FABP and L-FABP at results revealed that there were the biggest activity of Lb-FABP acid. This study provides a good foundation for the investigation protein level.

关 键 词：鸡 肝脏基础型脂肪酸结合蛋白 肝脏脂肪酸结合蛋白 结合属性 

分 类 号：S851.347[农业科学—预防兽医学]