产ACC脱氨酶植物根际促生菌的筛选及其对修复植物高羊茅生长的影响  

Isolation of Plant Growth Promoting Rhizobacteria Produced ACC Ammonialyase and Its Influence on Tall Fescue Growth

作　　者：丁琳琳[1,2] 刘五星[2] 孙剑英[1,2] 骆永明[2] 徐旭士[1] 李振高[2] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,江苏省微生物资源产业化工程技术研究中心,南京210046 [2]中国科学院土壤环境与污染修复重点实验室(南京土壤研究所),南京210008

出　　处：《土壤》2013年第2期271-276,共6页Soils

基　　金：国家自然科学基金项目(41001182);环境保护公益性行业科研专项基金项目(201009015)资助

摘　　要：以1．氨基环丙烷-1-羧酸（ACC）为唯一氮源，从石油污染的土壤中分离得到一株具有ACC脱氨酶活性的菌株D5A。该菌在以ACC为唯一氮源条件下，其ACC脱氨酶比活力为0．084U／mg。另外，D5A还具有产生吲哚乙酸（IAA）、耐盐以及溶磷的特性。在液体培养条件下该菌株产IAA高达112mg／L，在90g／kg盐含量的培养基中仍能够正常生长且具有较强的溶解矿物磷能力。同时该菌对酸碱具有良好的适应性，在初始pH4～10的LB培养基中生长良好。种子发芽试验表明在3g／kg和6g／kg浓度NaCl的逆境条件下，该菌株能显著提高高羊茅种子的发芽率和芽长。最后，通过对其进行生理生化特性和16SrDNA序列分析，该菌株初步鉴定为克雷伯氏菌（Klebsiellasp．）。In this study, the strains with 1-aminocyclopropane-l-carboxylic acid （ACC） deaminase activity were isolated from an oil-polluted soil. The ACC deaminase activity of the strain D5A was 0.084 U/mg in minimal medium containing ACC as the sole nitrogen source. In addition, D5A also had properties of producing indole acetic acid （IAA）, salt tolerance and dissolving phosphorus. In liquid culture conditions, the strains produced IAA up to 112 mg/L. It was still able to grow normally in the medium of 90 g/kg salt and had a strong ability to dissolve the mineral phosphorus. The strains grew well in LB medium from the initial pH value of 4 to 10, which showed the strains had good adaptability of pH. Seed germination tests showed that the strains could significantly improve seed germination and bud of tall fescue（Festuca arundinacea） at adverse conditions of 3 g/kg and 6 g/kg NaCI. Finally, based on physiological, biochemical characteristics and 16S rDNA sequence analysis, the strains was identified as Klebsiella sp.

关 键 词：高羊茅 ACC脱氨酶 IAA 铁载体 溶磷 

分 类 号：Q939.96[生物学—微生物学]