白及抑菌作用及其活性部位的初步研究  

Research on Antibacterial Activity and Substance of Bletilla striata(Thund.) Reichb.f.

作　　者：彭芙[1,2] 万峰[2,3] 熊亮[2,3] 彭成[2,3] 陈放[1] 

机构地区：[1]四川大学生命科学学院,四川成都610225 [2]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室中药材标准化教育部重点实验室,四川成都610075 [3]成都中医药大学药学院,四川成都610075

出　　处：《时珍国医国药》2013年第5期1061-1063,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家“十一五”科技支撑计划重点项目(No.2009BAI84B00)

摘　　要：目的探讨白及的抑菌作用及其活性部位。方法规范提取白及乙酸乙酯部位、正丁醇部位、萃取后水部位、醇提后水煎液部位4个部位,采用琼脂平板二倍稀释法测定以上4个部位对10个不同种属24株病原菌的MIC。结果乙酸乙酯部位和正丁醇部位对受试病原菌均具有广谱抑菌活性,4个部位中尤以乙酸乙酯部位的抑菌活性最强;乙酸乙酯部位对革兰阳性菌明显较对革兰阴性菌的抑菌活性强,对革兰阳性菌的MIC为0.065～1.042 mg/ml,对革兰阴性菌的MIC为2.083～8.333mg/ml。结论白及具有确切的体外广谱抑菌活性,乙酸乙酯部位是其主要的活性部位。Objective To study antibacterial activity of Bletilla striata（Thund.） Reichb.f.and the main component contributing to antibacterial activity. MethodsMinimum inhibitory concentrations（MICs） of ethyl acetate extract,butyl alcohol extract,water extract after extraction and water extract after ethanol extraction from Bletilla striata（Thund.） Reichb.f against 24 isolates were determined by agar dilution method. ResultsBoth ethyl acetate extract and butyl alcohol extract showed broad-spectrum antibacterial activity.Ethyl acetate extract had the highest activity among the 4 extracted fractions of Bletilla striata（Thund.） Reichb.f.Ethyl acetate extract obviously had higher activity against Gram-positive than Gram-negative isolates,with MIC ranging from 0.065～1.042 mg/ml against Gram-positive isolates,2.083～8.333 mg/ml against Gram-negative isolates,respectively. ConclusionBletilla striata（Thund.） Reichb.f.has broad-spectrum antibacterial activity indeed with ethyl acetate extract as the main antibacterial substance.

关 键 词：白及 抑菌作用 活性部位 

分 类 号：R285.5[医药卫生—中药学]