四君子汤含药血清与氟尿嘧啶合用对胃癌细胞SGC-7901增殖抑制作用研究  

Experimental Study on Sijunzi Decoction Medicated Serum and Fluorouracil on Inhibiting the Cycle of Growth and the Index of Apoptosis of SGC-7901 cells

作　　者：黄浏姣[1] 赵刚[1] 毛明[2] 

机构地区：[1]湖北中医药大学,湖北武汉430061 [2]武汉大学中南医院,湖北武汉430071

出　　处：《时珍国医国药》2013年第5期1236-1238,共3页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省自然科学基金(No.2005ABA190)

摘　　要：目的观察四君子汤含药血清和氟尿嘧啶(FU)合用对体外培养的人胃癌细胞SGC-7901的增殖及凋亡的影响。方法将低、中、高3种剂量的四君子汤含药血清与FU合用分别作用于人胃癌细胞SGC-7901,另设空白对照组和FU单用组,共计5个组,药物作用时间均为24h。采用流式细胞仪检测各组SGC-7901细胞周期分布和凋亡指数。结果 FU对SGC-7901细胞具有G1/S期阻滞作用;高剂量血清与FU合用具有明显的S期阻滞作用。AV/PI双染色法计数显示含药血清与FU合用组,细胞凋亡率分别与对照组比较有显著性差异,且较两药单用组的作用显著增强,且呈现剂量依赖性。结论四君子汤能增强FU对人胃癌细胞SGC-7901增殖的抑制和诱导凋亡作用。Objective To observe the efficacy of Sijunzi decoction with medicated serum and fluorouracil in inhibiting the cycle of growth and the index of apoptosis of gastric carcinoma cell. MethodsFive groups were set for the study as：FU combined high,medium,low dose groups,non-medicared serum group,and the FU group.The human gastric carcinoma cell line SGC-7901 were treated with Sijunzi decoction medicated serum combined with FU for 24 hours respectively.Flow cytometric detection of Sijunzi decoction with medicated serum and FU with different doses on gastric carcinoma cell cycle distribution and apoptosis index. ResultsMost cells were arrested in G1/S phase by FU used single and arrested in S phase by the serum containing SJZD of high dosage and FU used together group.AV/PI double staining method of counting,show the apoptotic index of Sijunzi decoction medicated serum combined with FU were significant differences with the control group;and more than two drugs when used alone,and the increase of dose effect stronger. ConclusionSJZD can enhanced the apoptosis and the proliferation inhibitive effect of SGC-7901 cells.

关 键 词：四君子汤 氟尿嘧啶 SGC-7901细胞 增殖 凋亡 

分 类 号：R289.5[医药卫生—方剂学]