海巴戟总黄酮提取工艺的响应面法优化  

Optimization of Extraction Conditions of Flavonoids from Morinda citrifolia by Response Surface Methodology

作　　者：赵俊凌[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所云南分所,云南景洪666100

出　　处：《时珍国医国药》2013年第5期1249-1251,共3页Lishizhen Medicine and Materia Medica Research

基　　金：中央级公益性科研院所基本科研业务专项(No.YZYN 201104)

摘　　要：目的采用响应面法对海巴戟总黄酮的提取工艺进行优化。方法在单因素实验的基础上,以乙醇浓度、提取温度、提取时间及料液比为自变量,总黄酮含量为响应值,研究各因素及其交互作用对总黄酮得率的影响。结果最优工艺条件为,乙醇浓度95%,液料比为1∶30 g.ml-1,提取温度为87.4℃,浸提时间为120 min,提取实际值为13.991 mg.g-1。结论采用响应面法对海巴戟黄酮提取条件进行优化合理可行。Objective Response surface methodology was used to optimize the extraction conditions of flavonoids from Morinda citrifolia. MethodsBased on one factor test,the individual and interactive effects of ethanol concentration,material/liquid ratio,extracting temperature and extracting time on the yield of flavonoids were studied. ResultsThe experiments showed that the optimal extracting conditions were as follows：ethanol concentration 95%,material/liquid ratio 1：30,extraction temperature 87.4℃ and extraction time 2 h.Under these conditions,the yield of flavonoids was 13.991mg.g-1. ConclusionThis exhibits the good feasibility of Response surface methodology for optimizing extracting process of flavonoids from Morinda citrifolia.

关 键 词：海巴戟 总黄酮 提取工艺 响应面法 

分 类 号：R284.2[医药卫生—中药学]