葡萄籽原花青素对镉致肝损伤保护作用的研究  

Study on protective effect of grape seed proanthocyanidins on liver damage induced by cadmium

作　　者：邹志辉[1] 杨翠婵[1] 李伯灵[1] 

机构地区：[1]广东药学院公共卫生学院,广州市510310

出　　处：《职业与健康》2013年第12期1418-1420,共3页Occupation and Health

基　　金：广东省中医药局项目"MAPKs信号在葡萄籽原花青素影响DNA损伤中的作用研究"(项目编号2010417)

摘　　要：目的探讨葡萄籽原花青素(GSP)对氯化镉(CdCl2)诱导大鼠肝氧化损伤的保护作用。方法 40只SPF级SD大鼠,雌雄各半,随机分为对照组(腹腔注射生理盐水和灌胃生理盐水)、染镉组(腹腔注射1.5 mg/kg的CdCl2和灌胃生理盐水)、GSP低、中、高剂量干预组(均腹腔注射1.5 mg/kg CdCl2,同时分别灌胃20、40、80 mg/kg GSP),采用硫代巴比妥酸法和单细胞凝胶电泳技术(SCGE)分别检测肝细胞中丙二醛(MDA)的含量和DNA损伤情况。结果染镉组肝细胞MDA含量和细胞彗尾长、Olive尾矩均明显高于对照组,表明1.5 mg/kg CdCl2可引发大鼠肝脏出现明显的脂质过氧化损伤。GSP抑制染镉大鼠肝细胞MDA的产生,呈明显的剂量-效应关系,GSP低、中、高剂量组MDA清除率分别为12.74%、24.22%、43.65%。GSP低、中、高剂量干预组中肝脏细胞的彗尾长、Olive尾矩均低于染镉组的,表明GSP具有一定抑制DNA损伤和促进DNA修复的生物学作用。结论 GSP对镉致肝组织脂质过氧化和DNA损伤有一定的保护作用。[ Objective] To explore the protective effect of grape seed proanthocyanidins （GSP） on CdC12-induced hepatic oxidative damage in rats. [ Methods ] 40 SD rats of SPF grade, half male and half female, were randomly divided into 5 groups, including the normal control group （ intraperitoneal injection and gastric perfusion of normal saline at the same dosage ） , CdC12 exposure group （ intraperitoneal injection of 1.5 mg/kg CdC12 and gastric perfusion of normal saline at the same dosage } , as well as low, middle and high-dose GSP intervention group （ intraperitoneal injection of 1.5 mg/kg CdC12, and gastric perfusion of 20,40 and 80 mg/kg GSP, respectively）. MDA content and DNA oxidative damage in hepatic cell was measured by thiobarbituric acid （TBA） method and single cell gel eletrophoresis （SCGE） respectively. [ Results ] MDA content, comet tail length and 0live tail moment in hepatic cell of the CdC12 exposure group were significantly higher than those of the normal control group, which indicated that CdC12 at a dosage of 1.5 mg/kg can induce an obvious hepatic oxidative damage in rat. GSP could inhibit the increase of MDA production, which showed an obvious dose-effect relationship. The clearances of MDA in low, middle and high-dose GSP intervention group was 12.74%, 24.22% and 43.65%, respectively. The comet tail length and Olive tail moment in hepatic cell of low, middle and high-dose GSP intervention group were lower than those of CdC12 exposure group, which indicated GSP may inhibit DNA damage or promote DNA repair. [ Conclusion] A certain dosage of GSP could antagonize CdC12-induced hepatic oxidative damage in rats.

关 键 词：葡萄籽原花青素 镉 肝 DNA损伤 

分 类 号：R114[医药卫生—卫生毒理学]