金华鼠类标本中检测到伯氏疏螺旋体DNA片段并测序分析  

Infection of Lyme spirochetes in small mammals in Jinhua, Zhejiang province

作　　者：叶晓东[1] 郑寿贵[1] 姜理平[2] 

机构地区：[1]浙江省金华市金东区疾病预防控制中心,金华321015 [2]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2013年第5期1057-1059,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解金华鼠类中伯氏疏螺旋体自然感染的状况。方法:用巢式PCR扩增鼠中的伯氏疏螺旋体5S-23srRNA间隔区片段,对阳性产物进行测序。结果:在浙江省金华捕到的128只鼠类标本中检到阳性12份,其中,黄毛鼠、小林姬鼠、鼩鼱阳性率分别为7.5%、1.43%和25%,其测序分析结果显示,这些动物感染的螺旋体与法雷斯疏螺旋体BorreliavalaisianaOS115(AB091447)和B.valaisianaQX13P25(DQ188933)核酸序列高度同源,相似率达100%。结论:金华地区存在伯氏疏螺旋体病原,可感染黄毛鼠、姬鼠、鼩鼱等宿主动物。Objective：To learn the infection of Lyme spirochetes in small mammals in Jinhua,Zhejiang province.Methods：Small mammals were captured with trap-night methods and the infections were investigated with nest PCRtargeting the 5S-23s rRNA internal space regions.DNA sequences of positive spirochetes infection in small mammalswere analysis and blast in Gene bank.Results： In total 128 small mammals sample,12 were found infected withLyme spirochetes and the infection rate of Rattus losea 、Apodemus sylvaticus Sorex spp.were 7.5%,1.43%and 25%respectively.The sequences of spirochetes were founded the same and shown high identity with Borrelia valaisiana OS115（AB091447）and B.valaisiana QX13P25（DQ188933） 。 Conculsion： The infection of Lyme spirochetes in R.losea 、A.sylvaticus and Sorex spp.indicate the health risk in this area.

关 键 词：鼠类 伯氏疏螺旋体 聚合酶反应 序列分析 

分 类 号：R514[医药卫生—内科学]