针刺对血管性痴呆大鼠血清TNF-α、IL-1β及学习记忆能力的影响  

Effects of Acupuncture on the Levels of TNF-α and IL-1β in Serum and on the Learning and Memory Ability of Vascular Dementia Rats

作　　者：陈英华[1] 孙忠人[2] 金弘[1] 洪霞[2] 姜磊[2] 秦瑞奇[2] 魏巍[2] 倪国忠[2] 

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《针灸临床杂志》2013年第5期56-60,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江中医药大学"优秀创新人才支持计划"科研项目;编号:2012 RCL01

摘　　要：目的:探讨针刺对血管性痴呆(VD)大鼠血清TNF-α、IL-1β及学习记忆能力的影响,为临床治疗血管性痴呆提供依据。方法:将168只SD雄性大鼠随机分为5组,即:正常组、假手术组、模型组、手针组、电针组。采用四血管阻断法制作VD大鼠模型。造模成功后,进行针刺四神聪、风池穴干预。从不同时间点通过Morris水迷宫测试VD大鼠的学习记忆能力;采用酶联免疫吸附法检测大鼠血清TNF-α、IL-1β含量的变化。结果:同一时间点,手针组、电针组与模型组比较,大鼠逃避潜伏期明显缩短,穿越平台次数增多,差异均具有统计学意义(P<0.05或P<0.01);同一时间点,电针组较手针组穿越平台次数明显增多,差异有统计学意义(P<0.05)。同一时间点,手针组、电针组大鼠血清TNF-α和IL-1β含量与模型组比较明显降低,差异具有统计学意义(P<0.01)。结论:针刺四神聪、风池穴能改善VD大鼠的学习记忆能力,具有脑保护作用,能降低VD大鼠血清中的TNF-α、IL-1β含量,并且电针比手针效果显著。Objective： To explore the effects of acupuncture on the levels of TNF-α and IL-1β in serum and on the ability of learning and memory in vascular dementia （VD） rats, to provide the basis for the clinical treat- ment of vascular dementia. Methods： 168 male SD rats were randomly divided into five groups, including nor- mal group, sham operation group, model group, acupuncture group and electro - acupuncture group. VD rat model was made with the four - vessel occlusion method. After successful modeling and Fengchi points, to test learning and memory in rats at different time points by changes , acupuncture on Sishencong Morris water maze ; to detect of TNF-α,IL-1β content levels in serum with the enzyme- linked immunosorbent assay. Results： At the same time point, the acupuncture group, EA group compared with model group, the rats escape latency was significantly shorter and the times of over - stepping of platform was increased, and the differences were sta- tistically significant（P 〈0.05 or P 〈0.01 ） ;at the same time point, the times of over- stepping of platform of EA group was significantly increased than those in acupuncture group, and the difference was statistically signifi-cant（P 〈0.05）. At the and EA group compared significant（ P 〈0.01 ） ; at same time point, TNF-α and IL-1β content levels in serum of acupuncture group with model group were significantly decreased, and the differences were statistically the same time point, TNF - ctcontent levels in serum of EA group puncture group was significantly decreased, and the difference was statistically significant（ P 〈 0.05）. Conclu- sion： Acupuncture on Sishencong and Fengchi can improve learning and memory ability of VD rats, and it has a protective effect on brain. Moreover, it can also reduce TNF-α and IL-1β contents in serum of VD rats, so e- lectro- acupuncture is more significant.

关 键 词：血管性痴呆 针刺 学习记忆能力 细胞因子 

分 类 号：R246.6[医药卫生—针灸推拿学]