辽东楤木协同顺铂抑制SKOV3-DDP生长的作用及机理  

Cooperative Effect of Aralia Elata(Miq.) Seem with Cisplatin and the Possible Mechanism

作　　者：王春梅[1] 薛晓鸥[2] 张广美[3] 

机构地区：[1]北京中医药大学东直门医院中西医结合博士后流动站,北京100700 [2]北京中医药大学东直门医院,北京100700 [3]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001

出　　处：《中医药信息》2013年第3期24-28,共5页Information on Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金(D200952);黑龙江省自然科学基金重点项目(ZD201110)

摘　　要：目的:探讨辽东楤木叶总皂苷与顺铂对卵巢癌耐药细胞株的协同抑制作用及机理。方法:采用MTT法检测细胞活性,Annexin-V FITC/PI法检测细胞凋亡,RT-PCR法检测LRP mRNA水平的表达,蛋白印迹法检测Phospho-iκB-α、Phospho-iκκα、Phospho-NF-κBp65蛋白质水平的表达。结果:辽东楤木叶总皂苷与顺铂可明显协同抑制SKOV3-DDP细胞生长,并诱导SKOV3-DDP细胞凋亡,同时下调耐药蛋白LRP mRNA水平的表达及NF-κB信号传导通路关键蛋白IκB-α、Iκ、NF-κBp65磷酸化的水平。结论:辽东楤木叶总皂苷与顺铂可协同抑制卵巢癌耐药细胞株的生长,这种作用可能与其诱导凋亡、降低耐药因子的表达有关,其作用机理可能是通过对NF-κB信号传导通路的阻断而实现的。Objective ： To detect the cooperative effect of TALAS with Cisplatin on the growth of SKOV3 - DDP cells, and to investigate the possible mechanism. Methods： We verified the inhibitory effect of TALAS on SK- OV3 - DDP cells previously. In this experiment, MTT assay was used to detect the cooperative effect of TA- LAS and Cisplatin, the apoptosis inducing effect of TALAS was investigated by Annexin - V FITC/PI, the levels of LRPmRNA were examined by RT - PCR, and the protein expressions of Phospho - iκB-α, Phospho - iκκα, Phospho - NF - κBp65 were detected by Western blot. Results ： TALAS showed an obvious cooperative inhibitory effect with Cisplatin on SKOV3 - DDP ceils. TALAS could induce apoptosis of SKOV3 - DDP ceils, the high and middle doses of TALAS showed a significant difference to control. Moreover, TALAS could down -regulate the mRNA expressions of LRP, meanwhile, the protein levels of Phospho- iκB -α, Phospho- iκκα, Phospho - NF - κBp65 were down - regulated by TALAS. Conclusion ： TALAS shows an obvious cooperative effect with Cisplatin possibly through inducing apoptosis and down - regulating the expressions of LRP. Moreover, it may be related to the inhibitory effect of TALAS on NF - κB signaling pathway.

关 键 词：辽东楤木 铂类 耐药 NF—κB 

分 类 号：R285.5[医药卫生—中药学]