丹红注射液及其活性组分对脂多糖诱导的小胶质细胞NO分泌的抑制作用  

Inhibition of Danhong Injection and its active components on NO secretion in microglia induced by lipopolysaccharide

作　　者：景浩然[1] 胡利民[1] 王少峡[1] 杨红云[1] 韩虞[1] 王鸿远[1] 

机构地区：[1]天津中医药大学中医药研究院天津市中药药理学重点实验室,教育部方剂学重点实验室,天津300193

出　　处：《现代药物与临床》2013年第3期285-287,共3页Drugs & Clinic

基　　金：国家重大新药创制科技重大专项(2012ZX09101201;2012ZX09101202;2011ZX09201-201);天津市卫生局课题(2010KZ120)

摘　　要：目的研究丹红注射液及其单体成分对小鼠小胶质细胞株(BV-2细胞)的影响及其作用机制。方法实验分为对照组、脂多糖(LPS)刺激组、加药组,检测不同稀释倍数的丹红注射液、水和醋酸乙酯萃取物及其单体成分对小胶质细胞活力及LPS诱导小胶质细胞一氧化氮(NO)产生的影响。结果丹红注射液及醋酸乙酯萃取物对LPS诱导的小胶质细胞NO的产生没有抑制作用,但是丹红注射液水萃取物以及丹酚酸A可以在不影响细胞活力的情况下抑制NO产生。结论丹红注射液水萃取物中的某些成分以及丹酚酸A抑制激活小胶质细胞NO的产生可能是丹红注射液神经保护作用的机制之一。Objective To study the effect and its mechanism of action of Danhong Injection and its monomer components on microglia BV-2 of mice.Methods The experiment was divided into control,lipopolysaccharide（LPS）,and dosing groups.The effects of Danhong Injection,water extract,ethyl acetate extract,and monomer compounds on microglia cell viability and LPS-induced NO production were detected.Results Danhong Injection and its ethyl acetate extract had no inhibitory effect on NO production of LPS-induced microglia,but water extract of Danhong Injection and its active component salvianolic acid A affected NO production without affecting cell viability.Conclusion The monomer components from Danhong Injection and salvianolic acid A inhibition NO production in activating microglia may be one of the neuroprotective effect mechanisms for neuroprotection of Danhong Injection.

关 键 词：丹红注射液 丹酚酸A 小胶质细胞 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]