不同保存条件下白刺属植物基因组DNA的提取  

Effects of Different Storage Conditions on Total DNA Extraction from Nitraria L.

作　　者：张玲[1,2] 王旭哲[3] 李先勇[1] 李志军[1,2] 舒尊哲[1,4,5] 

机构地区：[1]新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室,新疆阿拉尔843300 [2]塔里木大学植物科学学院,新疆阿拉尔843300 [3]塔里木大学动物科学学院,新疆阿拉尔843300 [4]塔里木大学生命科学学院,新疆阿拉尔843300 [5]郑州大学化学与分子工程学院,河南郑州450001

出　　处：《郑州大学学报（理学版）》2013年第2期99-103,共5页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家科技基础条件平台建设子项目;编号2005DK21006;兵团国际科技合作项目;编号2010YD37;兵团科技攻关计划项目;编号2011AB015;塔里木大学校长基金硕士项目;编号TDZKSS07001

摘　　要：以硅胶干燥的白刺叶片为材料,分别在-84℃、-20℃冰箱保存和常温保存,对三种不同保存条件下的白刺叶片进行DNA的提取,结果表明,在-84℃和-20℃冰箱里保存1～2年的材料均可采用3×CTAB法有效去除多糖、酚及蛋白质,可获得高质量基因组DNA,而常温保存半年的材料无法获得DNA.通过进行ISSR-PCR实验,提取的DNA扩增均可得到稳定、清晰、多态性好的PCR扩增图谱,-84℃保存的材料所得到的DNA在ISSR-PCR扩增中条带数目和清晰度明显优于-20℃保存的材料.The effects of total DNA extraction from Nitraria leaves dried with silica gel were studied under three different conditions for storing samples （ normal temperature and frozen at - 20 ℃and - 84℃ ） with improved 3 × CTAB method. It was suggested that the genomic DNA with high purity could be obtained after being frozen at - 20 ℃ or - 84 ℃ for one or two years. The polysaccharide, phenol and protein could be removed effectively. However the leaves at usual temperature could not provide the genomic DNA. Further results also showed that the leaf stored at - 84 ℃ was more suitable than the leaf stored at -20℃ for generating good ISSR-PCR banding patterns.

关 键 词：白刺属 保存方法 DNA提取 

分 类 号：Q943[生物学—植物学]