白细胞介素-1α和白细胞介素-6基因多态性与椎间盘退变症相关性研究  

Correlation research on gene polymorphism of interleukin-1α, interleukin-6 and intervertebral disc degeneration disease

作　　者：段雄 安菊霞 赵岩[2] 杨学军[2] 

机构地区：[1]乌兰察布医学高等专科学校附属医院外科,内蒙古乌兰察布012000 [2]内蒙古医科大学第二附属医院脊柱外科,内蒙古呼和浩特010058

出　　处：《中国医药导报》2013年第17期37-39,共3页China Medical Herald

基　　金：内蒙古自治区自然科学基金项目(编号200508010908)

摘　　要：目的研究白细胞介素-1α和白细胞介素-6基因多态性与椎间盘退变症相关性。方法选择2009年6月～2012年1月乌兰察布医学高等专科学校附属医院外科手术治疗的椎间盘退变症患者42例为病例组,选择同期因骨折和外伤行手术治疗的非椎间盘退变症患者85例为对照组。采用PCT-RFLP分析IL-1α-rs1800587和IL-6-rs1800797基因频率分布;采用多变量Logistic回归分析模型分析IL-1α-rs1800587和IL-6-rs1800797基因多态性对椎间盘退变的影响。结果①病例组IL-1α-rs1800587基因型中C/C、C/T和T/T基因型分布百分比为52.4%、40.5%和7.1%,而对照组相应基因型分布百分比为55.3%、38.8%和5.9%,两组比较,差异无统计学意义(P=0.87)。病例组IL-6-rs1800797基因型中A/A、A/G和G/G基因型分布百分比为50.0%、33.3%和16.7%,对照组相应基因型分布百分比为64.7%、28.2%和7.1%,两组比较,差异无统计学意义(P=0.17)。②IL-1α-rs1800587基因多态性并不能增加椎间盘退变的概率,携带IL-1α-rs1800587 C/T和T/T基因型调整后的OR值(95%CI)分别为1.28(0.18～7.27)和1.43(0.27～8.06)。多变量Logistic回归分析表明,IL-6-rs1800797 A/G基因多态性与椎间盘退变有相关关系。携带IL-6-rs1800797 G/G基因型的个体患椎间盘退变的概率显著高于携带IL-6-rs1800797 A/A基因型的个体,调整后的OR值(95%CI)为5.52(1.24～18.21)。结论 IL-6 rs1800797基因多态性对椎间盘退变发病过程有显著影响,检测基因多态性的可以预测椎间盘退变发病风险。Objective To research the correlation on gene polymorphism of interleukin-1α,interleukin-6 and intervertebral disc degeneration disease.Methods 42 patients with intervertebral disc degeneration disease treated by surgery from June 2009 to January 2012 in Affiliated Hospital of Wulanchabu Medical College were selected as cases group,85 patients without intervertebral disc degeneration disease treated surgery because of the fracture and trauma were chosen as control group.Gene frequency distribution of IL-1α-rs1800587 and IL-6-rs1800797 were analyzed by PCT-RFLP;influence of gene polymorphism of IL-1α-rs1800587 and IL-6-rs1800797 on intervertebral disc degeneration were analyzed by multivariable Logistic regression analysis model.Results ①Distribution percentage of C/ C,C/T,T/T genotype in IL-1α-rs1800587 were 52.4%,40.5%,7.1% respectively in cases group,corresponding genotype were 55.3%,38.8%,5.9% in control group,the differences were not statistically significant（P = 0.87）.Distribution percentage of A/A,A/G,G/G genotype in IL-6-rs1800797 were 50.0%,33.3%,16.7% respectively in cases group,corresponding genotype were 64.7%,28.2%,7.1% in control group,the differences were not statistically signifi cant（P = 0.17）.②Gene polymorphism of IL-1α-rs1800587 was found no function in increasing the probability of intervertebral disc degeneration disease,the OR（95%CI） value in C/T,T/T genotype were 1.28（0.18-7.27） and 1.43（0.27-8.06） in IL-1α-rs1800587.Correlationship was found between A/G gene polymorphism in IL-6-rs1800797 and intervertebral disc degeneration disease according to multivariable Logistic regression analysis.Probability of interver tebral disc degeneration disease in G/G genotype of IL-6-rs1800797 was higher than that in A/A genotype of IL-6rs1800797,the OR（95%CI） value was 5.52（1.24-18.21）.Conclusion IL-6 rs1800797 gene polymorphism has obvious influence in intervertebral disc degeneration disease,the detection of gene polymorphism can predict the risk of ntervertebral dis

关 键 词：白细胞介素-1Α 白细胞介素-6 椎间盘退变 发病风险 

分 类 号：R681.5[医药卫生—骨科学]