油菜BnRCH基因提高转基因拟南芥的耐盐性研究  

The studies of gene BnRCH from Brassica napus enhances the tolerance to salt stress in transgenic Arabido psis

作　　者：陈凤莲[1] 刘志斌[1] 王健美[1] 李旭锋[1] 杨毅[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2013年第3期643-648,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30971816;31171586);中央高校基本科研业务费资助(2011SCU11107)

摘　　要：为探索本实验室从甘蓝型油菜中克隆到的BnRCH基因在植物耐盐中的作用,比较了NaCl胁迫下转基因与野生型拟南芥在萌发及幼苗生长的差异.结果表明:在100 mMNaCl处理下,BnRCH转基因拟南芥种子萌发率比野生型高3～5倍;盐胁迫后野生型拟南芥幼苗首先表现出枯萎、白化现象;除去盐胁迫后,转基因拟南芥幼苗恢复生长状况明显优于野生型.本实验结果表明BnRCH基因能够提高转基因拟南芥的耐盐性.In order to explore the role of BnRCH,the unknown gene BnRCH was cloned from Brassica napus in our laboratory. Against salt stress, the differences between wild type and BnRCH transgenic Arabidopsis were studied under the NaCl stress. The results showed that, the germination rate of transgenic plants was three to five tolds higher than wild-type under 100mM NaCl. After salt stress, the wild-type seedlings firstly turned yellow and withered while the transgenic seedlings were slightly injured. When salt stress was removed, the recovery ability of the transgenic seedlings was obviously improved compared with wild-type. These studies suggested that BnRCH may paly an important role in enhancing the tolerance of salt stress.

关 键 词：盐胁迫 BnRCH 拟南芥 萌发率 幼苗生长 

分 类 号：Q78[生物学—分子生物学]