Immobilization of Hemoglobin on Cobalt Nanoparticles-modified Indium Tin Oxide Electrode： Direct Electrochemistry and Electrocatalytic Activity  

作　　者：LI Shuo-qi HU Jing-bo 

机构地区：[1]College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, P. R. China [2]College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China

出　　处：《Chemical Research in Chinese Universities》2013年第3期563-567,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（No.20211130505） and the Fundamental Research Funds for the Central Universities of China.

摘　　要：A cobalt nanoparticles-attached indium tin oxide（CoNPs/ITO） electrode was applied to the immobilization of hemoglobin（Hb） and an Hb modified CoNPs/ITO electrode（Hb/CoNPs/ITO） was prepared. The direct electron transfer of Hb was shown by the well-behaved voltammetric responses for Hb/CoNPs/ITO electrode and the effects of scan rate and pH value were observed. Based on the catalytic activity of Hb immobilized on the CoNPs/ITO electrode toward the reduction of H2O2, a mediator-free H2O2 sensor was developed. A linear relationship existed between the catalytic current and the H2O2 concentration in a range of 1.0--100.0 μmol/L with a detection limit （S/N=3） of 0.2μmol/L.A cobalt nanoparticles-attached indium tin oxide（CoNPs/ITO） electrode was applied to the immobilization of hemoglobin（Hb） and an Hb modified CoNPs/ITO electrode（Hb/CoNPs/ITO） was prepared. The direct electron transfer of Hb was shown by the well-behaved voltammetric responses for Hb/CoNPs/ITO electrode and the effects of scan rate and pH value were observed. Based on the catalytic activity of Hb immobilized on the CoNPs/ITO electrode toward the reduction of H2O2, a mediator-free H2O2 sensor was developed. A linear relationship existed between the catalytic current and the H2O2 concentration in a range of 1.0--100.0 μmol/L with a detection limit （S/N=3） of 0.2μmol/L.

关 键 词：Cobalt nanopartiele Indium tin oxide electrode Direct electron transfer HEMOGLOBIN ELECTROCATALYSIS Hydrogen peroxide 

分 类 号：O646.5[理学—物理化学] TN304.21[理学—化学]