山香圆叶中黄酮苷类成分及其抗炎活性研究  

Flavonoid glycosides from leaves of Turpinia arguta and their anti-inflammatory activity

作　　者：马双刚[1] 袁绍鹏[1] 侯琦[1] 李勇[1] 陈霞[1] 庾石山[1] 

机构地区：[1]天然药物活性物质与功能国家重点实验室,中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《中国中药杂志》2013年第11期1747-1750,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2012ZX09301002-002)

摘　　要：应用大孔树脂、聚酰胺、Sephadex LH-20,HPLC等各种色谱技术对山香圆叶95%乙醇提取物经乙酸乙酯萃取后的水部位进行分离纯化,分离得到8个黄酮苷类化合物,采用NMR等谱学方法分别鉴定为:野漆树苷(1),女贞苷(2),刺槐素-7-O-[β-D-吡喃葡萄糖基-(1→6)-α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(3),刺槐素-7-O-[α-L-吡喃鼠李糖基-(1→6)-α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(密蒙花新苷,4),木犀草素-7-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(5),金圣草素-7-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(6),刺槐素-7-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(蒙花苷,7),芹菜素6,8-二-C-β-D-吡喃葡萄糖苷(8)。其中化合物3～8为首次从该植物中分离获得。采用脂多糖(LPS)刺激RAM264.7巨噬细胞炎症模型对分离得到的化合物进行抗炎活性评价,结果表明化合物2,3在1×10-5mol.L-1浓度下,对LPS刺激RAW264.7细胞NO生成的抑制率分别为33.7%和23.8%,提示化合物2,3具有较弱的抗炎活性。Eight compounds were isolated from the leaves of Turpinia arguta by various chromatograhic techniques such as D101 macroporous resin,polyamide,Sephadex LH-20,and HPLC chromatography,and their structures were elucidated as rhoifolin（1）,apigenin-7-O-[2″-O-α-L-rhamnopyranosyl-6″-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside（2）,acacetin-7-O-[2″-O-α-L-rhamnopyranosyl-6″-O-β-D-glucopyranosyl]-β-D-glucopyranoside（3）,acacetin-7-O-[2″-O-α-L-rhamnopyranosyl-6″-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside（neobudofficide, 4）,luteolin-7-O-[2″-O-β-D-glucopyranosyl]-β-D-glucopyranoside（5）,chrysoeiml-7-O-[2″-O-β-D-glucopyranosyl]-β-D-glucopyranoside（6）,acacetin-7-O-α-L-rhamnopyranosyl-（1→6）-O-β-D-glucopyranoside（buddleoside,linarin, 7）, and apigenin 6, 8-di-C-β-D-glucopyranoside（8）on the basis of spectral data analysis. Compounds 3-8 were isolated from T. arguta for the first time. Compounds 2, 3 showed weak anti-inflammatory effect on LPS-stimulated RAW264.7 cell.

关 键 词：山香圆叶 黄酮苷 抗炎活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]