白芍水提部位化学成分的HPLC-ESI-Q-TOF-MS分析  

Analysis on chemical components from water extract of Paeoniae Radix Alba by high performance liquid chromatography-electrospray ionizationquadrupole-time of flight-mass spectrometry

作　　者：孙东东[1] 徐晓芳[2] 崔九成[2] 宋小妹[2] 李祥[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]陕西中医学院药学院,陕西咸阳712046

出　　处：《中国中药杂志》2013年第11期1760-1765,共6页China Journal of Chinese Materia Medica

基　　金：江苏高校优势学科建设工程项目(2011ZYX6-014);江苏省2012年度普通高校研究生科研创新计划项目(CXZZ12-0626)

摘　　要：该实验通过HPLC-ESI-Q-TOF-MS技术对白芍水提取部分中的化学成分进行定性分析。采用C18色谱柱(Han-bon LichrospherTM,4.6 mm×250 mm,5μm),以甲醇-0.1%甲酸溶液为流动相梯度洗脱;质谱使用ESI离子源,正离子与负离子模式下采集数据,通过分析正、负离子质谱信息及元素组成,并与相关文献数据比对,共鉴定出11个化合物。实验利用了HPLC的高效分离和MS的高灵敏检测优势,为阐明白芍水提部分的药效物质基础提供有力的依据。This experiment was performed to establish a qualitative analysis on chemical composition in water extract of Paeoniae Radix Alba by HPLC-ESI-Q-TOF-MS. The analysis was conducted on a C18 （Hanbon LichrospherTM, 4.6 mm×250 mm,5μm） column with methanol-0.1% formic acid as the mobile phase for gradient elution; ESI ion source was used for mass spectra, and data were collected in both positive and negative modes. The results showed that eleven compounds from water extract of Paeoniae Radix Alba had been identified by analyzing positive and negative ion mass data including element composition and by comparing with data from literatures. Since efficient separation of HPLC and the high sensitive detection of MS was used, this experiment, it will provide evidences for elucidation of the effective substance in the water extract of Paeoniae Radix Alba

关 键 词：白芍 化学成分 HPLC—ESI—Q-TOF—MS 

分 类 号：R284[医药卫生—中药学]