川楝子中肝毒性成分的快速筛查研究  

Study on rapid screening and identifying hepatotoxic compounds in Toosendan Fructus

作　　者：赵筱萍[1,2] 葛志伟[3] 张玉峰[3] 兰小红[2] 张伯礼[1] 

机构地区：[1]天津中医药大学,天津300193 [2]浙江中医药大学,浙江杭州310053 [3]浙江大学药学院,浙江杭州310058

出　　处：《中国中药杂志》2013年第11期1820-1822,共3页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2011ZX09307-002-01);浙江省科技计划项目(2008C23065)

摘　　要：使用荧光探针FDA标记的HepG2细胞模型及细胞荧光显微图像自动分析法,对川楝子的23个化学组分进行快速筛查,发现5个组分具有明显毒性。对其中的2个组分进行液质联用分析,推测鉴定了10个化学成分,制备并鉴定出其中3个成分的分子结构(meliasenin B,trichilinin D,1-O-tigloy-1-O-debenzoylohchinal)。进一步实验研究发现,这3个成分对HepG2细胞呈量-毒关系,提示川楝子中这些成分可能引起肝毒性。Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MSn, and 3 of them （meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal） were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.

关 键 词：川楝子 HEPG2细胞 中药毒性成分 毒性成分快速筛查 肝毒性 

分 类 号：R284[医药卫生—中药学]