一株刺盘孢属真菌中新的倍半萜及其细胞毒活性研究  

A new sesquiterpenoid from fungus Colletotrichum sp. and its cytotoxicity

作　　者：杨志钧[1] 杨天[1] 罗敏玉[2] 夏兴[1] 陈代杰[1] 钱秀萍[1] 

机构地区：[1]上海交通大学药学院,上海200040 [2]上海来益生物药物研究开发中心,上海200240

出　　处：《药学学报》2013年第6期891-895,共5页Acta Pharmaceutica Sinica

基　　金：国家重大新药创制(2010ZX09401-403)

摘　　要：为了研究刺盘孢属真菌的大米发酵产物的细胞毒活性成分,对其进行了化学研究和细胞毒活性分析。从其乙酸乙酯浸提部分分离得到4个化合物,经波谱数据分析,鉴定为新的倍半萜collectotriol(1)和已知化合物isoaltenuene(2)、altenuene(3)和4,10-二甲氧基链格孢酚(4)。细胞毒活性表明,化合物1～4对肺癌细胞A549、乳腺癌细胞MDA-MB-231和肝癌细胞PANC-1有一定的细胞毒活性;化合物4会使PANC-1细胞膨大而死亡,在IC50(60.2μg·mL?1)浓度下未能诱导细胞凋亡。A novel sesquiterpenoid （1） and three known compounds identified as isoaltenuene （2）, altenuene （3）, and alternariol 4, 10-O-dimethyl ether （4）, were isolated in our investigation of the cytotoxic constituents from solid cultures of the endophytic fungus Colletotrichum sp. The structures of these compounds were elucidated through spectroscopic data analysis. All compounds exhibited cytotoxic activity against lung cancer cell line A549, breast cancer cell line MDA-MB-231 and pancreatic cancer cell line PANC-1. Compound 4 could induce the PANC-1 cells inflation or death, but couldn＇t induce apoptosis at the IC50 of 60.2μg.mL-1.

关 键 词：刺盘孢属 倍半萜 细胞毒活性 细胞凋亡 

分 类 号：R284[医药卫生—中药学]