电针“夹脊”穴对心肌缺血再灌注损伤大鼠心肌细胞凋亡及凋亡调控基因表达的影响  

Effects of electroacupuncture at "Jiaji"(EX-B 2) on myocardial apoptosis and the expression of Bcl-2 and Bax in rats with ischemia-reperfusion injury

作　　者：孔素平[1] 张昕[2] 谭奇纹[2] 

机构地区：[1]山东中医药大学附属医院针灸科,济南250011 [2]山东中医药大学针灸推拿学院,济南250011

出　　处：《中国针灸》2013年第6期529-532,共4页Chinese Acupuncture & Moxibustion

基　　金：山东省自然科学基金项目:ZR2010 HL 064

摘　　要：目的:探讨针刺对心肌缺血再灌注损伤的保护机制。方法:将50只Wistar大鼠随机分成5组:假手术组、模型组、电针夹脊组、电针内关组、电针阳陵泉组,每组10只。后4组采用结扎左冠状动脉前降支的方法复制动物模型。假手术组不结扎冠状动脉。各治疗组分别电针刺激双侧"夹脊""内关""阳陵泉"穴。每日1次,连续治疗7d。假手术组与模型组不予治疗。采用TUNEL法检测心肌细胞凋亡,免疫组化法检测B淋巴细胞瘤-2基因(Bcl-2)、B细胞淋巴瘤因子相关X蛋白(Bax)表达。结果:(1)电针夹脊组、电针内关组和假手术组中细胞凋亡指数(AI)明显低于模型组;其他各组AI均高于假手术组(P<0.01);电针夹脊组与电针内关组AI值差异无统计学意义(P>0.05)。(2)电针夹脊组、电针内关组与模型组相比,Bcl-2蛋白表达量显著升高(P<0.01),而Bax蛋白表达显著下降(P<0.01);其他各组Bcl-2、Bax蛋白表达量均高于假手术组(P<0.01);电针夹脊组和电针内关组中的Bcl-2、Bax值差异无统计学意义(P>0.05)。结论:电针夹脊穴、内关穴均对缺血再灌注损伤心肌有明显的保护作用,其机制可能与通过调节Bcl-2、Bax等基因的表达从而抑制细胞凋亡密切相关。ABSTRACT Objective To explore the protective mechanism of acupuncture on myocardial ischemia-reperfusion injury. Methods Fifty Wistar rats were randomly divided into a sham-operation group, a model group, a Jiaji group , a Neiguan group and a Yanglingquan group, 10 rats in each group. The model of myocardial ischemia- reperfusion injury was duplicated by ligating the left anterior descending coronary artery （LADCA） in the later four groups, and the LADCA was not ligated in the sham-operation group. The rats in the treatment groups were treated with electroacupuncture at“Jiaji” （EX-B 2）, “Neiguan” （PC 6） and “Yanglingquan” （GB 34） on both sides, respectively, once a day for 7 days. No interventions were produced in the sham-operation group and model group. Myocardial apoptosis were examined by the TUNEL method. The expressions of Bcl-2 and Bax proteins were measured by immunohistochemical method. Results Apoptosis index（AI） was significantly lower in the Jiaji group, Neiguan group and sham-operation group compared with model group （P〈0. 01）. Compared with the sham-operation group, AI were significantly increased in other groups （all P〈0. 01）. There was no significant difference in AI between Jiaji and Neiguan group （P〉0.05）. Compared with the model group, the expression of Bcl-2 was significantly increased and Bax protein was significantly decreased in Jiaji group and Neiguan group （both P〈0.01）. The expressions of Bcl-2 and Bax proteins were significantly increased in other groups compared with the sham-operation group （all P〈0.01）. There was no significant difference in the expression of Bcl-2 and Bax proteins between Jiaji and Neiguan group （P〉0.05）. Conclusion Electroacupuncture at both Jiaji （EX-B 2） and Neiguan （PC 6） has protective effects on myocardial isehemia-reperfusion injury, and the mechanism is closely re- lated to inhibiting myocardial apoptosis by adjusting the expression of Bcl-2 and Bax.

关 键 词：心肌缺血再灌注 电针 细胞凋亡 B淋巴细胞瘤-2基因 B细胞淋巴瘤因子相关X蛋白 

分 类 号：R245.9[医药卫生—针灸推拿学]